Stomata respond slowly to changes in light when compared with photosynthesis, undermining plant water-use efficiency (WUE). We know much about stomatal mechanics, yet efforts to accelerate stomatal responsiveness have been limited despite the breadth of potential targets for manipulation. Here, we use mechanistic modeling to establish a hierarchy of putative targets affecting stomatal kinetics. Counterintuitively, modeling predicted that overexpressing plasma membrane H + -ATPases could speed stomata and enhance WUE under fluctuating light, even though overexpressed H + -ATPases is known to promote stomatal opening and reduce WUE in the steady state. Experiments validated the prediction, implicating an unexpected role of the H + -ATPases in improving WUE under fluctuating light. It suggests that H + -ATPases have a dual function, acting as a facilitator of carbon assimilation and water use, depending on the light conditions. These findings highlight the importance of integrating in silico modeling with experiments in future efforts toward enhancing stomatal function.

中文翻译：

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过表达质膜 H + -ATP 酶在平衡碳水利用中的双重功能


与光合作用相比，气孔对光的变化反应缓慢，破坏了植物水分利用效率 （WUE）。我们对气孔机制了解很多，但尽管潜在的操纵目标很广，但加速气孔反应性的努力一直受到限制。在这里，我们使用机理建模来建立影响气孔动力学的假定靶标的层次结构。与直觉相反，建模预测过表达质膜 H + -ATP 酶可以在波动的光线下加速气孔并增强 WUE，即使已知过表达的 H + -ATP 酶会促进气孔打开并降低稳态下的 WUE。实验验证了这一预测，表明 H + -ATP 酶在波动光下改善 WUE 方面具有意想不到的作用。它表明 H + -ATP 酶具有双重功能，根据光照条件，充当碳同化和水利用的促进剂。这些发现强调了将计算机建模与未来增强气孔功能的实验相结合的重要性。