The gastrointestinal diseases caused by Helicobacter pylori (H. pylori) infection made the accurate detection of H. pylori infection more important. Non-invasive methods, such as molecular diagnostic methods, had become a promising method for detection of H. pylori. Stool samples combined with loop-mediated isothermal amplification (LAMP), showed potential practicability for real-time detection. However, complex nucleic acid extraction steps were required to remove the large numbers of amplification inhibitors in stool samples before LAMP reaction. And the limited number of H. pylori made the detection with long reaction time and low sensitivity. The problems mentioned above were urgently to be solved.

中文翻译：

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水凝胶环介导的等温扩增，无需核酸提取即可超快速诊断粪便样本中的幽门螺杆菌


幽门螺杆菌 （H. pylori） 感染引起的胃肠道疾病使得幽门螺杆菌感染的准确检测变得更加重要。非侵入性方法，例如分子诊断方法，已成为检测幽门螺杆菌的一种很有前途的方法。粪便样本结合环介导等温扩增 （LAMP） 技术，显示出实时检测的潜在可行性。然而，在 LAMP 反应之前，需要复杂的核酸提取步骤来去除粪便样品中的大量扩增抑制剂。幽门螺杆菌数量有限，因此检测反应时间长，灵敏度低。上述问题亟待解决。