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Impact of blue light on cutaneous barrier structures and properties: NPLC/HR-MSn and Raman analyses
Analyst ( IF 3.6 ) Pub Date : 2024-11-05 , DOI: 10.1039/d4an01244e Léa Habib, Rime Michael-Jubeli, Marie Abboud, Roger Lteif, Ali Tfayli
Analyst ( IF 3.6 ) Pub Date : 2024-11-05 , DOI: 10.1039/d4an01244e Léa Habib, Rime Michael-Jubeli, Marie Abboud, Roger Lteif, Ali Tfayli
Skin health relies heavily on a well-maintained cutaneous barrier. While the detrimental effects of UV radiation on the epidermis are established, the impact of blue light, a significant component of sunlight and artificial sources, is less clear. This study aims to explore blue light's influence on the reconstructed human epidermis (RHE) using two complementary analytical approaches: Raman microspectroscopy and normal phase liquid chromatography coupled with high-resolution mass spectrometry (NPLC/HR-MSn). RHE samples were exposed to blue light (415 nm and 455 nm) during different stages of their maturation. Raman spectra were acquired for both irradiated and non-irradiated (control) samples. Raman descriptors were analyzed to assess potential alterations in the structural organization of proteins and lipids’ conformational changes. Additionally, lipids from RHE samples were extracted and analyzed using NPLC/HR-MSn. Blue light exposure led to changes in the structural organization of RHE lipids and proteins, as well as changes in the lipid composition. These changes varied depending on the wavelength and exposure dose. Exposure to blue light could disrupt the integrity of the skin's protective barrier, leading to increased sensitivity to environmental stressors and potential skin damage.
中文翻译:
蓝光对皮肤屏障结构和特性的影响:NPLC/HR-MSn 和拉曼分析
皮肤健康在很大程度上依赖于维护良好的皮肤屏障。虽然紫外线辐射对表皮的有害影响已经确定,但蓝光(阳光和人工光源的重要组成部分)的影响尚不清楚。本研究旨在使用两种互补的分析方法探讨蓝光对重建人表皮 (RHE) 的影响:拉曼显微光谱和正相液相色谱-高分辨率质谱 (NPLC/HR-MSn)。RHE 样品在其成熟的不同阶段暴露在蓝光 (415 nm 和 455 nm) 下。采集了辐照和非辐照(对照)样品的拉曼光谱。分析拉曼描述符以评估蛋白质结构组织的潜在改变和脂质的构象变化。此外,还提取了 RHE 样品中的脂质,并使用 NPLC/HR-MSn 进行分析。蓝光照射导致 RHE 脂质和蛋白质结构组织的变化,以及脂质组成的变化。这些变化因波长和暴露剂量而异。暴露在蓝光下可能会破坏皮肤保护屏障的完整性,导致对环境压力源和潜在皮肤损伤的敏感性增加。
更新日期:2024-11-06
中文翻译:
蓝光对皮肤屏障结构和特性的影响:NPLC/HR-MSn 和拉曼分析
皮肤健康在很大程度上依赖于维护良好的皮肤屏障。虽然紫外线辐射对表皮的有害影响已经确定,但蓝光(阳光和人工光源的重要组成部分)的影响尚不清楚。本研究旨在使用两种互补的分析方法探讨蓝光对重建人表皮 (RHE) 的影响:拉曼显微光谱和正相液相色谱-高分辨率质谱 (NPLC/HR-MSn)。RHE 样品在其成熟的不同阶段暴露在蓝光 (415 nm 和 455 nm) 下。采集了辐照和非辐照(对照)样品的拉曼光谱。分析拉曼描述符以评估蛋白质结构组织的潜在改变和脂质的构象变化。此外,还提取了 RHE 样品中的脂质,并使用 NPLC/HR-MSn 进行分析。蓝光照射导致 RHE 脂质和蛋白质结构组织的变化,以及脂质组成的变化。这些变化因波长和暴露剂量而异。暴露在蓝光下可能会破坏皮肤保护屏障的完整性,导致对环境压力源和潜在皮肤损伤的敏感性增加。