ScopeAging in males can lead to declines in testosterone production, essential for maintaining male reproductive health.Methods and resultsTo investigate the effects of dietary supplementation with alpha‐linolenic acid (ALA) on testosterone production in aged breeder roosters and understand the underlying molecular mechanisms involved. An in vivo model is established to investigate the effects of dietary ALA supplementation on testosterone production in aged breeder roosters, and the Leydig cell culture is used to identify the potential molecular mechanism. Dietary supplementation with ALA increases in plasma testosterone. Congruently, ALA supplementation enhances the expression of testosterone biosynthesis‐related enzymes. ALA supplementation exerts anti‐apoptotic effects in testicular mitochondria, as evidenced by a lower expression of pro‐apoptotic factors and a higher expression of the anti‐apoptotic factor B‐cell lymphoma 2 (Bcl‐2). Moreover, In Leydig cells, ALA supplementation promotes mitochondrial biogenesis genes. The proposed mechanism is that ALA activates the sirtuin1 (SIRT1) pathway and is supported by higher SIRT1 transcript and protein in Leydig cells. Furthermore, blocking SIRT1 with siRNA reverses ALA's effects on testosterone biosynthesis and mitochondrial function‐related genes.ConclusionThese findings indicate that dietary supplementation with ALA can improve testosterone production in aged breeder roosters, possibly by modulation of mitochondrial function via activating the SIRT1 pathway.

中文翻译：

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补充 α-亚麻酸可改善老年种鸡模型中的睾酮产生：线粒体调节和 SIRT1 激活的作用


范围男性衰老会导致睾丸激素分泌下降，而睾丸激素的产生对维持男性生殖健康至关重要。方法和结果研究膳食补充剂 α-亚麻酸 （ALA） 对老年种鸡睾酮产生的影响，并了解所涉及的潜在分子机制。建立了体内模型来研究膳食中 ALA 补充剂对老年种鸡睾酮产生的影响，并使用 Leydig 细胞培养物来确定潜在的分子机制。膳食补充 ALA 会增加血浆睾酮。同时，ALA 补充剂增强了睾酮生物合成相关酶的表达。ALA 补充剂在睾丸线粒体中发挥抗凋亡作用，促凋亡因子的表达较低和抗凋亡因子 B 细胞淋巴瘤 2 （Bcl-2） 的表达较高证明了这一点。此外，在 Leydig 细胞中，ALA 补充剂可促进线粒体生物发生基因。提出的机制是 ALA 激活 sirtuin1 （SIRT1） 通路，并得到 Leydig 细胞中较高 SIRT1 转录本和蛋白的支持。此外，用 siRNA 阻断 SIRT1 可逆转 ALA 对睾酮生物合成和线粒体功能相关基因的影响。结论这些发现表明，膳食中添加 ALA 可以改善老年种鸡的睾酮产生，可能是通过激活 SIRT1 通路调节线粒体功能。