Metabolic exchanges between strains in gut microbial communities shape their composition and interactions with the host. This study investigates the metabolic synergy between potential probiotic bacteria and Saccharomyces boulardii, aiming to enhance anti-inflammatory effects within a multi-species probiotic community. By screening a collection of 85 potential probiotic bacterial strains, we identified two strains that demonstrated a synergistic relationship with S. boulardii in pairwise co-cultivation. Furthermore, we computationally predicted cooperative communities with symbiotic relationships between S. boulardii and these bacteria. Experimental validation of 28 communities highlighted the role of S. boulardii as a key player in microbial communities, significantly boosting the community’s cell number and production of anti-inflammatory effectors, thereby affirming its essential role in improving symbiotic dynamics. Based on our observation, one defined community significantly activated the aryl hydrocarbon receptor—a key regulator of immune response—280-fold more effectively than the community without S. boulardii. This study underscores the potential of microbial communities for the design of more effective probiotic formulations.

中文翻译：

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布拉酵母菌通过益生菌群落中的代谢相互作用增强抗炎效应子和 AhR 激活


肠道微生物群落中菌株之间的代谢交换决定了它们的组成以及与宿主的相互作用。本研究调查了潜在益生菌和布拉酵母菌之间的代谢协同作用，旨在增强多物种益生菌群落内的抗炎作用。通过筛选 85 种潜在益生菌菌株的集合，我们确定了两种菌株，它们在成对共培养中表现出与布拉氏链球菌的协同关系。此外，我们计算预测了布拉氏链球菌与这些细菌之间具有共生关系的合作群落。对 28 个群落的实验验证强调了布拉氏链球菌在微生物群落中的关键作用，显着增加了群落的细胞数量和抗炎效应物的产生，从而肯定了它在改善共生动力学方面的重要作用。根据我们的观察，一个明确的群落显著激活芳烃受体（免疫反应的关键调节因子）的效率是没有布拉氏链球菌的群落的 280 倍。这项研究强调了微生物群落在设计更有效的益生菌配方方面的潜力。