Chemical Synthesis of HMGA1a Proteins with Post-translational Modifications via Ser/Thr Ligation,Organic Letters

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Chemical Synthesis of HMGA1a Proteins with Post-translational Modifications via Ser/Thr Ligation
Organic Letters ( IF 4.9 ) Pub Date : 2016-11-04 00:00:00 , DOI: 10.1021/acs.orglett.6b03056
Tianlu Li ₁ , Heng Liu ₁ , Xuechen Li ₁

Affiliation

The first chemical synthesis of nuclear protein HMGA1a via Ser/Thr ligation is reported. Notably, Hmb (2-hydroxy-4-methoxybenzyl) exhibits crucial improvement of both the difficult coupling during solid phase peptide synthesis and the poor ligation encountered in protein synthesis. These efforts led to preparation of HMGA1a analogs with well-defined phosphorylation and methylation patterns (9 synthetic proteins in total), thus overcoming the heterogeneous and combinatory problems inherent to protein post-translational modifications (PTMs), and facilitating the study of the regulatory roles of such PTMs.

中文翻译：

通过Ser / Thr连接进行翻译后修饰的HMGA1a蛋白的化学合成

据报道，通过Ser / Thr连接进行了核蛋白HMGA1a的首次化学合成。值得注意的是，Hmb（2 - h ydroxy -4- m ethoxy b enzyl）对固相肽合成过程中的困难偶联和蛋白质合成中的不良连接均显示出关键性的改善。这些导致制剂HMGA1a的努力类似物具有良好限定的磷酸化和甲基化模式（总共9个合成的蛋白），因此克服了非均相和组合子固有的问题蛋白p ost-吨ranslational米odifications（翻译后修饰），和促进的研究此类PTM的监管作用。

更新日期：2016-11-04

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