Cell membranes are vital barriers that regulate the composition of the intracellular environment and facilitate communication processes essential for cellular function and survival. In comparison to lipid membranes, artificial polymeric membranes generally offer enhanced stability due to their higher molecular weight and greater variability in the nature of the macromolecular building blocks, which provides access to a broad chemistry toolbox to regulate important features such as fluidity and permeability. We recently developed an artificial cell platform based on a complex coacervate, in which a terpolymer, composed of a hydrophilic poly(ethylene glycol) segment, a hydrophobic poly(caprolactone-g-trimethylene carbonate) domain and a polyglutamate anchor (PEG-PCLgTMC-PGA) was used for stabilization. These membranized structures showed excellent permeability, due to the high fluidity of the membrane. However, the polymer membrane proved to be unselective with regard to the molecular weight of guest molecules that were exchanged with the environment. To advance this platform, a series of terpolymers with distinctive features were synthesized to further refine their regulatory features of the polymer membrane. Through investigation of structural terpolymer variants, including those in which the hydrophobic domain was based on PCLgTMC, poly(D,L-lactic acid) or polystyrene, their influence on membrane permeability, fluidity, and sequestration of hydrophobic molecules, such as cholesterol, was determined. With this extended range of membrane-forming building blocks, this coacervate platform is equipped with tailored permeability through interactions with the coacervate lumen and facilitates sequestration of hydrophobic molecules into the membrane and controlled fluidity.

中文翻译：

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控制合成三元共聚物稳定复合物凝聚物的膜流动性和生物物理特性


细胞膜是调节细胞内环境组成并促进对细胞功能和生存至关重要的通讯过程的重要屏障。与脂质膜相比，人造聚合物膜通常具有更高的稳定性，因为它们的分子量更高，并且大分子结构单元的性质具有更大的可变性，这为调节流动性和渗透性等重要特征提供了广泛的化学工具箱。我们最近开发了一种基于复杂凝聚物的人工细胞平台，其中使用由亲水性聚乙二醇链段、疏水性聚（己内酯-g-三亚甲基碳酸酯）结构域和聚谷氨酸锚 （PEG-PCLgTMC-PGA） 组成的三元共聚物进行稳定。由于膜的高流动性，这些膜化结构显示出优异的渗透性。然而，事实证明，聚合物膜对与环境交换的客体分子的分子量是无选择性的。为了推进该平台，合成了一系列具有独特特征的三元共聚物，以进一步完善其对聚合物膜的调节特征。通过研究结构三元聚合物变体，包括疏水结构域基于 PCLgTMC、聚（D，L-乳酸）或聚苯乙烯的变体，确定了它们对膜通透性、流动性和疏水分子（如胆固醇）隔离的影响。 凭借这种扩展的膜形成构建单元，该凝聚物平台通过与凝聚物腔的相互作用配备了定制的渗透性，并有助于疏水分子螯合到膜中并控制流动性。