A key restriction of label-free surface-enhanced Raman spectroscopy (SERS) in analysis of objects with complex composition (including with several target analytes) is the competition of mixture components for interaction with SERS-active surface. This leads to poor selectivity of the analysis of such mixtures (e.g., body fluids) and the need to use advanced sample pretreatment procedures such as HPLC or TLC. Therefore, this work aims to develop a set of simple and fast pretreatment steps (dilution, pH correction, etc.) to increase the sorption of the target analyte, reduce the sorption of admixtures, and prevent suppression of the target analyte SERS signal.

中文翻译：

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使用无标记 SERS 结合简单的样品预处理测定尿液中的多种分析物


在分析具有复杂成分的物体（包括几种目标分析物）时，无标记表面增强拉曼光谱 （SERS） 的一个主要限制是混合物组分与 SERS 活性表面相互作用的竞争。这导致此类混合物（例如体液）的分析选择性差，并且需要使用先进的样品预处理程序，例如 HPLC 或 TLC。因此，这项工作旨在开发一套简单快速的预处理步骤（稀释、pH 校正等），以增加目标分析物的吸附，减少外加剂的吸附，并防止抑制目标分析物 SERS 信号。