An integrated membrane filtration system was developed to make water purity suitable for microbiology/molecular biology research. Water samples were collected from outlets in different buildings of the National Research Center and analyzed for their composition before filtration. An integrated membrane system was developed based on mathematical modeling. Flat sheet membranes were produced, including microfiltration, ultrafiltration, and nanofiltration membranes. The flat sheet membranes were converted to a spiral wound module filter to simulate the local market filters and applied in the integrated membrane system that was designed and installed. The produced water was analyzed and compared to molecular-grade water used in different molecular biology/microbiology applications. Both PCR, agarose gel electrophoresis, bacterial liquid cultures, and viral propagation indicated that treated water using the herein-developed system exhibited comparable performance to the molecular grade water provided with imported reagent kits. So, this research can offer a promising solution for producing high-quality water suitable for sensitive laboratory applications.

开发了一种集成的膜过滤系统，使水的纯度适用于微生物学/分子生物学研究。从国家研究中心不同建筑物的出口收集水样，并在过滤前分析其成分。基于数学建模开发了一种集成膜系统。生产平板膜，包括微滤、超滤和纳滤膜。平板膜被转换为螺旋缠绕模块过滤器，以模拟当地市场的过滤器，并应用于设计和安装的集成膜系统。对采出水进行了分析，并与不同分子生物学/微生物学应用中使用的分子级水进行了比较。PCR、琼脂糖凝胶电泳、细菌液体培养和病毒增殖均表明，使用本文开发的系统的处理水表现出与进口试剂盒提供的分子级水相当的性能。因此，这项研究可以为生产适用于敏感实验室应用的高质量水提供一种有前途的解决方案。