Enzymes with ingenious structures and diverse functions are crucial for biomedical applications but face challenges like instability, limited targetability, and delivery complexity. We developed core-shell DNA-enzyme conjugates using rolling circle amplification (RCA), creating RCA-based DNA-enzyme nanostructure (RCA-DEN) for efficient enzyme immobilization and functionalization. RCA-DEN, characterized by densely packed nucleic acids and negligible disruption of enzyme activity, increases the stability of enzymes and nucleic acids while reducing technical difficulties, making it a versatile platform for diverse biomedical applications. This approach facilitates the modular customization of enzymes and the incorporation of functionalities such as aptamers and DNAzymes. The efficacy of RCA-DEN has been demonstrated in several areas, including selective catalysis, cascade catalysis, dynamic monitoring of intracellular chemical processes, and synergistic therapeutic interventions against tumors. Overall, this work provides a new perspective on enzyme immobilization and functionalization, paving the way for broader biomedical applications of enzymes.

中文翻译：

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基于滚环扩增的 DNA-酶纳米结构用于酶的固定和功能化


具有巧妙结构和多种功能的酶对于生物医学应用至关重要，但面临不稳定性、靶向性有限和递送复杂性等挑战。我们使用滚环扩增 （RCA） 开发了核壳 DNA-酶偶联物，创建了基于 RCA 的 DNA-酶纳米结构 （RCA-DEN），用于高效的酶固定和功能化。RCA-DEN的特点是核酸密集堆积，酶活性的破坏可以忽略不计，在降低技术难度的同时，增加了酶和核酸的稳定性，使其成为多种生物医学应用的多功能平台。这种方法有助于酶的模块化定制以及适配体和 DNA 酶等功能的整合。RCA-DEN 的疗效已在多个领域得到证实，包括选择性催化、级联催化、细胞内化学过程的动态监测以及针对肿瘤的协同治疗干预。总的来说，这项工作为酶固定和功能化提供了新的视角，为酶更广泛的生物医学应用铺平了道路。