Optimized methods for the synthesis of circular RNA in vitro and in cells, and a complementary Cas9 de-immunization method, enhance RNA persistence and reduce immunogenicity for applications in genome engineering and cell engineering.

中文翻译：

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用于 RNA 环化的扩展工具包


体外和细胞中环状 RNA 合成的优化方法，以及互补的 Cas9 去免疫方法，增强了 RNA 持久性并降低了免疫原性，可用于基因组工程和细胞工程。