The accurate reconstruction of genome-scale metabolic models (GEMs) for unculturable species poses challenges due to the incomplete and fragmented genetic information typical of metagenome-assembled genomes (MAGs). While existing tools leverage sequence homology from single genomes, this study introduces pan-Draft, a pan-reactome-based approach exploiting recurrent genetic evidence to determine the solid core structure of species-level GEMs. By comparing MAGs clustered at the species-level, pan-Draft addresses the issues due to the incompleteness and contamination of individual genomes, providing high-quality draft models and an accessory reactions catalog supporting the gapfilling step. This approach will improve our comprehension of metabolic functions of uncultured species.

中文翻译：

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pan-Draft：来自多个基因组的物种代表性代谢模型的自动重建


由于宏基因组组装基因组 （MAG） 典型的遗传信息不完整和碎片化，因此无法培养物种的基因组规模代谢模型 （GEM） 的准确重建带来了挑战。虽然现有工具利用来自单个基因组的序列同源性，但本研究引入了 pan-Draft，这是一种基于泛反应组的方法，利用递归的遗传证据来确定物种水平 GEM 的固体核心结构。通过比较在物种水平上聚集的 MAG，pan-Draft 解决了由于单个基因组的不完整和污染而导致的问题，提供了高质量的 Draft 模型和支持间隙填充步骤的辅助反应目录。这种方法将提高我们对未培养物种代谢功能的理解。