Elucidating organismal developmental processes requires a comprehensive understanding of cellular lineages in the spatial, temporal, and molecular domains. In this study, we introduce Zebrahub, a dynamic atlas of zebrafish embryonic development that integrates single-cell sequencing time course data with lineage reconstructions facilitated by light-sheet microscopy. This atlas offers high-resolution and in-depth molecular insights into zebrafish development, achieved through the sequencing of individual embryos across ten developmental stages, complemented by reconstructions of cellular trajectories. Zebrahub also incorporates an interactive tool to navigate the complex cellular flows and lineages derived from light-sheet microscopy data, enabling in silico fate-mapping experiments. To demonstrate the versatility of our multimodal resource, we utilize Zebrahub to provide fresh insights into the pluripotency of neuro-mesodermal progenitors (NMPs) and the origins of a joint kidney-hemangioblast progenitor population.

中文翻译：

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多模态斑马鱼发育图谱揭示了晚脊椎动物多能轴向祖细胞的状态转换动力学


阐明生物体发育过程需要全面了解空间、时间和分子领域的细胞谱系。在这项研究中，我们介绍了 Zebrahub，这是一种斑马鱼胚胎发育的动态图谱，它将单细胞测序时程数据与光片显微镜促进的谱系重建相结合。该图谱通过对十个发育阶段的单个胚胎进行测序，并辅以细胞轨迹的重建，为斑马鱼的发育提供了高分辨率和深入的分子见解。Zebrahub 还集成了一个交互式工具，用于导航从光片显微镜数据得出的复杂细胞流和谱系，从而实现计算机命运映射实验。为了展示我们多模式资源的多功能性，我们利用 Zebrahub 为神经中胚层祖细胞 （NMP） 的多能性和肾脏-血管母细胞联合祖细胞群的起源提供了新的见解。