BACKGROUND Cytochrome-P450 eicosanoids from arachidonic acid, including vasodilator epoxyeicosatrienoic acids (EETs) and the vasoconstrictor 20-HETE, are important in regulating blood pressure, vascular tone, and cardiac and renal function. This study examined plasma 20-HETE and EETs in relation to blood pressure and vascular stiffness in a cohort of 1054 community-dwelling young adults from the Raine Study at 27 years. METHODS Plasma 20-HETE, EETs, and their hydroxylated products were measured by liquid chromatography-tandem mass spectrometry. Pulse wave velocity and aortic distensibility were measured to assess vascular stiffness. Sex differences were assessed using univariate analysis. Multiple regression models assessed the relationship between 20-HETE and systolic blood pressure (SBP) and measures of vascular stiffness. RESULTS The regression model for SBP showed a positive relationship with plasma 20-HETE (β, 0.092; P<0.0001), after adjusting for sex (P<0.0001), body mass index (P<0.0001), (ln)triglycerides (P<0.0001), and (ln)Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) (P=0.015), and accounted for 29% of the variance in SBP. 20-HETE was positively related to pulse wave velocity (β, 0.059; P=0.021); after adjusting for sex (P<0.0001), SBP (P<0.0001), (ln)triglycerides (P=0.014), (ln)HOMA-IR (P=0.0001), (ln)high-sensitivity C-reactive protein (P=0.005), and age (P=0.002), the model accounted for 34% of the variance in pulse wave velocity. 20-HETE was inversely associated with aortic distensibility (β, -0.051; P=0.029), independent of sex (P<0.0001), SBP (P<0.0001), and (ln)HOMA-IR (P<0.0001); the model accounted for 25% of the variance in aortic distensibility. Plasma EETs were not significant predictors of SBP or vascular stiffness. CONCLUSIONS In young adults, 20-HETE may play a fundamental role in regulating blood pressure and vascular stiffness independent of numerous cardiometabolic risk factors and cytochrome 450-derived EETs. REGISTRATION URL: https://www.anzctr.org.au; Unique identifier: CTRN12617001599369.

中文翻译：

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20-HETE，年轻人的血压和血管僵硬。


背景 来自花生四烯酸的细胞色素 P450 类花生酸，包括血管扩张剂环氧二十碳三烯酸 （EET） 和血管收缩剂 20-HETE，在调节血压、血管张力以及心脏和肾功能方面很重要。这项研究检查了血浆 20-HETE 和 EETs，该队列由 Raine 研究的 1054 名社区居住的年轻人在 27 岁时与血压和血管僵硬的关系。方法 采用液相色谱-串联质谱法测量血浆 20-HETE 、 EETs 及其羟基化产物。测量脉搏波速度和主动脉扩张性以评估血管硬度。使用单变量分析评估性别差异。多元回归模型评估了 20-HETE 与收缩压 （SBP） 以及血管硬度测量之间的关系。结果 SBP 回归模型显示与血浆 20-HETE （β， 0.092;P<0.0001），调整性别 （P<0.0001）、体重指数 （P<0.0001）、（ln）甘油三酯 （P<0.0001） 和 （ln） 胰岛素抵抗稳态模型评估 （HOMA-IR） （P=0.015），并占 SBP 方差的 29%。20-HETE 与脉搏波速度呈正相关 （β， 0.059;P=0.021）;调整性别 （P<0.0001）、SBP （P<0.0001）、（ln）甘油三酯 （P=0.014）、（ln）HOMA-IR （P=0.0001）、（ln）高敏 C 反应蛋白 （P=0.005） 和年龄 （P=0.002） 后，模型占脉搏波速度方差的 34%。20-HETE 与主动脉扩张性呈负相关 （β，-0.051;P=0.029）、不分性别 （P<0.0001）、SBP （P<0.0001） 和 （ln）HOMA-IR （P<0.0001）;该模型占主动脉扩张性方差的 25%。 血浆 EET 不是 SBP 或血管僵硬的显着预测因子。结论 在年轻人中，20-HETE 可能在调节血压和血管僵化方面发挥重要作用，独立于众多心脏代谢危险因素和细胞色素 450 衍生的 EET。注册网址：https://www.anzctr.org.au;唯一标识符：CTRN12617001599369。