Oxidative stress is a crucial concept in redox biology, and significant progress has been made in recent years. Excessive levels of reactive oxygen species (ROS) can lead to oxidative damage, heightening vulnerability to various diseases. By contrast, ROS maintained within a moderate range plays a role in regulating normal physiological metabolism. Choosing suitable animal models in a complex research context is critical for enhancing research efficacy. While rodents are frequently utilized in medical experiments, they pose challenges such as high costs and ethical considerations. Alternatively, non-rodent model organisms like zebrafish, Drosophila, and C. elegans offer promising avenues into oxidative stress research. These organisms boast advantages such as their small size, high reproduction rate, availability for live imaging, and ease of gene manipulation. This review highlights advancements in the detection of oxidative stress using non-rodent models. The oxidative homeostasis regulatory pathway, Kelch‐like ECH‐associated protein 1-Nuclear factor erythroid 2-related factor 2 (Keap1-Nrf2), is systematically reviewed alongside multiple regulation of Nrf2-centered pathways in different organisms. Ultimately, this review conducts a comprehensive comparative analysis of different model organisms and further explores the combination of novel techniques with non-rodents. This review aims to summarize state-of-the-art findings in oxidative stress research using non-rodents and to delineate future directions.

中文翻译：

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有前途的氧化应激工具：非啮齿类模式生物综述


氧化应激是氧化还原生物学中的一个重要概念，近年来取得了重大进展。过量的活性氧 （ROS） 会导致氧化损伤，增加对各种疾病的脆弱性。相比之下，维持在中等范围内的 ROS 在调节正常的生理代谢中发挥作用。在复杂的研究环境中选择合适的动物模型对于提高研究效率至关重要。虽然啮齿动物经常用于医学实验，但它们带来了高成本和道德考虑等挑战。或者，斑马鱼、果蝇和秀丽隐杆线虫等非啮齿动物模式生物为氧化应激研究提供了有前途的途径。这些生物体具有体积小、繁殖率高、可进行实时成像和易于基因操作等优点。本综述重点介绍了使用非啮齿动物模型检测氧化应激的进展。系统评价了氧化稳态调节途径，Kelch 样 ECH 相关蛋白 1-核因子红细胞 2 相关因子 2 （Keap1-Nrf2），以及不同生物体中 Nrf2 中心途径的多种调节。最终，本综述对不同的模式生物进行了全面的比较分析，并进一步探讨了新技术与非啮齿动物的结合。本综述旨在总结使用非啮齿动物进行氧化应激研究的最新发现，并描绘未来的方向。