Antibacterial resistance is a severe threat to modern medicine and human health. To stay ahead of constantly-evolving bacteria we need to expand our arsenal of effective antibiotics. As such, antisense therapy is an attractive approach. The programmability allows to in principle target any RNA sequence within bacteria, enabling tremendous selectivity. In this Tutorial Review we provide guidelines for devising effective antibacterial antisense agents and offer a concise perspective for future research. We will review the chemical architectures of antibacterial antisense agents with a special focus on the delivery and target selection for successful antisense design. This Tutorial Review will strive to serve as an essential guide for antibacterial antisense technology development.

中文翻译：

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反义抗生素的化学策略


抗菌素耐药性是对现代医学和人类健康的严重威胁。为了领先于不断进化的细菌，我们需要扩大我们的有效抗生素库。因此，反义疗法是一种有吸引力的方法。可编程性原则上允许靶向细菌内的任何 RNA 序列，从而实现极高的选择性。在本教程回顾中，我们提供了设计有效抗菌抗义剂的指南，并为未来的研究提供了简明的视角。我们将回顾抗菌反义剂的化学结构，特别关注成功反义设计的递送和靶点选择。本教程回顾将努力成为抗菌反义技术开发的重要指南。