Thiocyanate (SCN⁻) exists in various industries and is detrimental to the ecosystem, necessitating cost-effective and environmentally benign treatment. In response to alleviate the bacterial toxicity of SCN⁻, this study developed a two-stage coupled system by tandem of anammox in reactor 1 (R1) and SCN⁻-driven autotrophic denitrification in reactor 2 (R2), achieving simultaneous removal of SCN⁻ and nitrogen. The total nitrogen removal efficiency of the coupled system was 92.42 ± 1.98%, with nearly 100% of SCN⁻ elimination. Thiobacillus was responsible for SCN⁻ degradation. The deduced degradation pathway of SCN⁻ was via the cyanate pathway before coupling, followed by the co-action of cyanate pathway and carbonyl sulfide pathway after coupling. Although scaling-up study is needed to validate its applicability in real-world applications, this study contributes to the advancement of sustainable and cost-effective wastewater treatment technologies, being an attractive path for low-carbon nitrogen removal and greenhouse gas emission-free technology.

硫氰酸盐 （SCN⁻） 存在于各个行业，对生态系统有害，需要具有成本效益和环境友好的处理。为了减轻 SCN⁻ 的细菌毒性，本研究开发了一种两阶段耦合系统，在反应器 1 （R1） 中串联使用厌氧氨氧化，在反应器 2 （R2） 中串联使用 SCN⁻ 驱动的自养反硝化作用，实现了 SCN⁻ 和氮的同时去除。耦合系统的总脱氮效率为 92.42 ± 1.98%，SCN⁻ 去除率接近 100%。硫杆菌负责 SCN⁻ 降解。推导的 SCN⁻ 降解途径在偶联前通过氰酸盐途径，然后是偶联后氰酸盐途径和羰基硫化物途径的共同作用。尽管需要扩大规模的研究来验证其在实际应用中的适用性，但这项研究有助于推进可持续和具有成本效益的废水处理技术，成为低碳脱氮和无温室气体排放技术的有吸引力的途径。