Ischemia and reperfusion states are studied in a network of cardiomyocytes as a part of real‐state conditions of heart injuries and inflammations, specifically myocardial infractions. Arrays of graphene field‐effect transistors (GFETs) fabricated in this work are used for extracellular recordings of ischemia states of cardiac cells during the external triggering of the ischemia infarction. The low‐concentrated ischemic buffer solution allows to create a cell‐stress condition resulting in the reperfusion process. The results show that the action potentials recorded with the graphene transistors, especially their shape, and duration of the active segment in measured extracellular action potentials, can be used to characterize the real state of the studied cardiac cell culture. The unique property of GFETs to detect such small changes in the action potential of cells in cardiac healthy and unhealthy states provides prospects for building the next generation of ultrasensitive biosensors, enabling the detection of acute ischemic states at an early stage.

中文翻译：

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石墨烯场效应晶体管用于早期心肌缺血的研究


缺血和再灌注状态在心肌细胞网络中进行研究，作为心脏损伤和炎症（特别是心肌梗死）真实状态条件的一部分。这项工作中制造的石墨烯场效应晶体管 （GFET） 阵列用于在缺血梗死的外部触发过程中心肌细胞缺血状态的细胞外记录。低浓度缺血缓冲溶液允许产生细胞应激条件，从而导致再灌注过程。结果表明，用石墨烯晶体管记录的动作电位，尤其是它们的形状和测量的细胞外动作电位中活动片段的持续时间，可用于表征所研究的心肌细胞培养物的真实状态。GFET 能够检测心脏健康和不健康状态下细胞动作电位的如此微小变化的独特特性为构建下一代超灵敏生物传感器提供了前景，从而能够在早期检测急性缺血状态。