The selection of the matrix is crucial for matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). This work successfully synthesized metal–organic framework (MOF) matrices to address the limitations on the application of traditional organic matrices in the study of small molecule compositions, and Ti-based MOF nanosheets were screened as matrices for imaging the hepatotoxic components of Polygonum multiflorum. Comparison between six MOF materials and traditional organic matrices showed that Ti-based MOF nanosheets have less background interference, significant stability, and high salt resistance. The imaging results indicated that the main components of Polygonum multiflorum, free anthraquinone and stilbene glycoside have unique spatial distribution characteristics. Successful application of the synthesized Ti-based MOF nanosheets in mass spectrometry imaging improved the detection ability of mass spectrometry imaging in the small molecule field, and spatiotemporal content changes of hepatotoxic components in Polygonum multiflorum during the steaming process were observed, providing a scientific basis for steaming.

中文翻译：

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Ti 基 MOF 纳米片作为低分子量化合物的质谱成像基质，揭示何首乌加工过程中肝毒性成分的时空含量变化


基质的选择对于基质辅助激光解吸电离质谱成像 （MALDI-MSI） 至关重要。这项工作成功合成了金属-有机框架 （MOF） 基质，以解决传统有机基质在小分子组成研究中应用的局限性，并筛选出 Ti 基 MOF 纳米片作为多花何首乌肝毒性成分成像的基质。六种MOF材料与传统有机基体的比较表明，Ti基MOF纳米片具有较少的背景干扰、显著的稳定性和高耐盐性。成像结果表明，何首乌的主要成分、游离蒽醌和二苯乙烯糖苷具有独特的空间分布特征。合成的Ti基MOF纳米片在质谱成像中的成功应用提高了质谱成像在小分子领域的检测能力，并观察到了蒸制过程中何首乌中肝毒性成分的时空含量变化，为蒸制提供了科学依据。