This study into the response of two Ipomoea batatas (L.) Lam cultivars, Melinda and Tio Joe, to coinfection with sweet potato chlorotic stunt virus (SPCSV) and sweet potato pakakuy virus (SPPV), employed a comprehensive approach encompassing symptomatology, real-time quantitative polymerase chain reaction, metagenomics, and transcriptomics. SPCSV is a quarantine virus with synergistic effects, which decimate yields. SPPV is the most prevalent DNA virus in sweet potato germplasm, with a tendency to persist in meristems, posing a significant risk for germplasm transfer between territories. Graft inoculation experiments revealed that while Tio Joe remained asymptomatic for 12 weeks and suppressed virus replication, Melinda displayed symptoms early on and exhibited high virus titers. Metagenomic analyses corroborated these observations and confirmed that SPCSV and SPPV were responsible. Transcriptomic analysis unveiled disparities in gene expression between Melinda and Tio Joe. Differential gene expression was heightened and altered in Melinda as the viruses disrupted its gene expression. Its defense strategies, such as inducing abscisic acid signaling, were insufficient to overcome disruptive viral effects like oxidative stress, rendering it susceptible. In contrast, Tio Joe had relatively stable differential gene expression, indicating resistance to SPPV–SPCSV coinfection. Overexpressed genes such as sirtuin, rapid alkalization factor, and nuclear pore anchor triggered quantitative resistance, supported with enriched organelles such as mitochondrion and pathways such as proteasome and cutin, suberine, and wax biosynthesis. Tio Joe maintained its genome integrity and inhibited viral replication by tightly controlling gene expression and preventing reactive oxygen species accumulation.

中文翻译：

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破解甘薯绿菌噱头和 pakakuy 病毒共感染的防御策略


本研究研究了两个 Ipomoea batatas （L.） Lam 品种 Melinda 和 Tio Joe 对甘薯绿化矽动病毒 （SPCSV） 和甘薯 pakakuy 病毒 （SPPV） 共同感染的反应，采用了包括症状学、实时定量聚合酶链反应、宏基因组学和转录组学在内的综合方法。SPCSV 是一种具有协同作用的检疫病毒，会大幅降低产量。SPPV 是甘薯种质中最常见的 DNA 病毒，倾向于在分生组织中持续存在，对种质在领土间转移构成重大风险。移植物接种实验显示，虽然 Tio Joe 在 12 周内保持无症状并抑制了病毒复制，但 Melinda 很早就表现出症状并表现出高病毒滴度。宏基因组分析证实了这些观察结果，并证实 SPCSV 和 SPPV 是原因。转录组学分析揭示了 Melinda 和 Tio Joe 之间基因表达的差异。随着病毒破坏其基因表达，Melinda 的差异基因表达增加和改变。它的防御策略，如诱导脱落酸信号传导，不足以克服氧化应激等破坏性病毒效应，使其易感。相比之下，Tio Joe 具有相对稳定的差异基因表达，表明对 SPPV-SPCSV 共感染具有耐药性。过表达基因（如 sirtuin、快速碱化因子和核孔锚）触发了定量抗性，并得到了丰富的细胞器（如线粒体）和途径（如蛋白酶体和角质、软木碱和蜡生物合成）的支持。 Tio Joe 通过严格控制基因表达和防止活性氧积累来保持其基因组完整性并抑制病毒复制。