ObjectivesThis study aimed to assess the effect of an oscillating chitosan brush (OCB) compared with an ultrasonic device with PEEK tip (US‐PEEK) for mechanical implant surface decontamination using an in vitro model combining 3D models and a validated dynamic multispecies biofilm.Materials and MethodsA multispecies biofilm using six bacterial strains (Streptococcus oralis, Veillonella parvula, Actinomyces naeslundii, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans) was seeded on dental implants with machined and sandblasted, large‐grit and acid‐etched (SLA) surfaces. These were installed in 3D models depicting peri‐implant defect. Mechanical decontamination was performed for 120 s using either an OCB or a US‐PEEK. A negative control group received no treatment. Scanning electron microscopy (SEM) was used to evaluate the bacterial composition and quantitative PCR (qPCR) analyzed the number of each bacterial species [colony‐forming units per milliliter (CFU/mL)].ResultsWell‐structured biofilms with a dense microbial distribution were observed on the negative control implants after 72 h. qPCR following mechanical decontamination showed a scarce bacterial reduction in the OCB group. The US‐PEEK group exhibited a significant decrease in bacterial species compared to both OCB and control groups (p < 0.05). A biofilm removal effect was also observed in the OCB group for the machined implant surfaces.ConclusionIn vitro assessment using an anatomical 3D model showed that mechanical decontamination effectively reduced biofilm. The US‐PEEK group demonstrated biofilm reduction on the SLA surface, while the OCB group showed a reduction on the machined implant surface. Additionally, the US‐PEEK group demonstrated greater efficacy in reducing bacterial numbers.

中文翻译：

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振荡壳聚糖刷与超声波 PEEK 尖端的去污效果比较：使用动态生物膜模型的体外研究


目的本研究旨在使用结合 3D 模型和经过验证的动态多物种生物膜的体外模型，评估振荡壳聚糖刷 （OCB） 与带有 PEEK 尖端的超声装置 （US-PEEK） 相比对机械植入物表面净化的效果。材料和方法使用六种细菌菌株 （口链球菌、小韦荣菌、放线菌、有核梭杆菌、牙龈卟啉单胞菌和放线菌聚集杆菌）的多物种生物膜接种在具有机加工和喷砂、大砂和酸蚀 （SLA） 表面的牙科植入物上。这些被安装在描绘种植体周围缺损的 3D 模型中。使用 OCB 或 US-PEEK 进行 120 秒的机械去污。阴性对照组未接受治疗。扫描电子显微镜 （SEM） 用于评估细菌组成，定量 PCR （qPCR） 分析每种细菌种类的数量 [每毫升菌落形成单位 （CFU/mL）]。结果72 小时后，在阴性对照植入物上观察到结构良好的生物膜，微生物分布致密。机械去污后的 qPCR 显示 OCB 组细菌稀少减少。与 OCB 组和对照组相比，US-PEEK 组的细菌种类显着减少 （p < 0.05）。在 OCB 组中还观察到机加工植入物表面的生物膜去除效果。结论使用解剖 3D 模型的体外评估表明，机械去污有效减少了生物膜。US-PEEK 组显示 SLA 表面的生物膜减少，而 OCB 组显示机加工植入物表面的生物膜减少。 此外，US-PEEK 组在减少细菌数量方面表现出更大的效果。