Essential oils (EOs) are a complex mix of chemical compounds and can have antibacterial activity. Thus, they can improve the delivery and effectiveness of antimicrobials and are promising candidates for the development of antimicrobial alternatives against resistant microorganisms. In this study, we investigated the chemical composition of Cinnamomum triplinerve essential oil (CtEO), as well as the in vitro and in vivo antimicrobial activity alone and in combination with amikacin (AMK). The results of gas chromatography and mass spectrometry (GCMS) analyses showed that the main components of CtEO were (E) – nerolidol (28.0 %) (the major compound), β–selinene (13.6 %), β–elemene (12.0 %), α–selinene (10.9 %), viridiflorol (6.4 %), (E)-caryophyllene (6.3 %), and spathulenol (4.8 %). In vitro, CtEO along with amikacin (AMK) showed synergistic activity against a polymyxin-resistant K. pneumoniae strain. CtEO + AMK can extravasate proteins and nucleic acids within 3 h of treatment. Effective concentrations showed hemocompatibility, no signs of toxicity in vivo in Caenorhabditis elegans and ensured the survival of infected nematodes. Our results suggested that the association of CtEO and AMK exhibited antimicrobial activity against K. pneumoniae; thus, it is a promising therapeutic alternative as an antimicrobial agent.

中文翻译：

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肉桂三酯精油作为阿米卡星载体剂对抗多粘菌素耐药的肺炎克雷伯菌菌株


精油 （EO） 是化合物的复杂混合物，具有抗菌活性。因此，它们可以改善抗菌剂的递送和有效性，并且是开发针对耐药微生物的抗菌替代品的有希望的候选者。在这项研究中，我们研究了肉桂三方精油 （CtEO） 的化学成分，以及单独和与阿米卡星 （AMK） 联合使用的体外和体内抗菌活性。气相色谱和质谱 （GCMS） 分析结果显示，CtEO 的主要成分为 （E） – 橙花醇 （28.0 %） （主要化合物）、β-硒烯 （13.6 %）、β-榄烯 （12.0 %）、α-硒烯 （10.9 %）、草胺花酚 （6.4 %）、（E）-石竹烯 （6.3 %） 和沙土烯醇 （4.8 %）。在体外，CtEO 与阿米卡星 （AMK） 一起显示出对多粘菌素耐药的肺炎克雷伯菌菌株的协同活性。CtEO + AMK 可在处理后 3 小时内外渗蛋白质和核酸。有效浓度显示血液相容性，秀丽隐杆线虫体内无毒性迹象，并确保受感染线虫的存活。我们的结果表明，CtEO 和 AMK 的结合表现出对肺炎克雷伯菌的抗菌活性;因此，它是一种很有前途的抗菌剂治疗替代品。