Docosahexaenoic acid (DHA), an essential omega-3 fatty acid, is crucial for the normal development and function of the brain. Its production can be achieved through the partial hydrolysis of DHA-rich triglycerides catalyzed by lipases, particularly from fish oils. In this work, the characterization of anchovy oil capsules revealed that they offer a pure and concentrated source of DHA, with more than 90 % of the oil in the form of triglycerides. Therefore, the hydrolysis reaction was studied with the aim of releasing 100 % of the DHA present in this oil, using different immobilized lipases with maximum enzyme loading on the hydrophobic support Immobeads-C18. The study’s results revealed that our immobilization strategy through hydrophobic adsorption improved the catalytic properties of activity and selectivity of the TLL lipase compared to other biocatalysts described in the literature. Additionally, complete hydrolysis of the oil was achieved in just 24 h with the NS40-C18 derivative, which could be reused up to 6 cycles without loss of activity.

中文翻译：

---

通过富含 Omega-3 的油的酶水解生产二十二碳六烯酸


二十二碳六烯酸 （DHA） 是一种必需的 omega-3 脂肪酸，对大脑的正常发育和功能至关重要。它的生产可以通过脂肪酶催化的富含 DHA 的甘油三酯的部分水解来实现，特别是从鱼油中。在这项工作中，鳀鱼油胶囊的表征表明，它们提供了纯净而浓缩的 DHA 来源，其中超过 90% 的油以甘油三酯的形式存在。因此，研究了水解反应，目的是释放该油中存在的 100% DHA，使用不同的固定化脂肪酶，在疏水支持物 Immobeads-C18 上具有最大的酶负载量。研究结果表明，与文献中描述的其他生物催化剂相比，我们通过疏水吸附的固定化策略改善了 TLL 脂肪酶活性和选择性的催化特性。此外，使用 NS40-C18 衍生物只需 24 小时即可实现油的完全水解，该衍生物最多可重复使用 6 个循环而不会失去活性。