<br>短短芽孢杆菌 BF19 的综合基因组分析揭示了其对苦瓜枯萎病的生物防治潜力,BMC Microbiology

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短短芽孢杆菌 BF19 的综合基因组分析揭示了其对苦瓜枯萎病的生物防治潜力
BMC Microbiology ( IF 4.0 ) Pub Date : 2024-10-18 , DOI: 10.1186/s12866-024-03519-7
Luyang Song ₁ , Yue Shen ₂ , Huihao Zhang ₁ , Han Zhang ₁ , Yuanyuan Zhang ₁ , Mengjiao Wang ₁ , Mingyue Zhang ₁ , Fei Wang ₃ , Lin Zhou ₁ , Caiyi Wen ₁ , Ying Zhao ₁

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苦瓜枯萎病是一种由土传病原体尖孢镰刀菌 f. sp. momordicae （FOM）引发的严重维管疾病，显着限制了苦瓜的产量。在本研究中，分离鉴定了短芽孢杆菌（Brevibacillus brevibacillus brevis）的一种新型菌株 BF19，通过体内和体外实验表现出对 FOM 的强抗菌活性。为了全面评估菌株 BF19 的生物防治潜力，我们进行了表型、系统发育和比较基因组学分析。表型分析表明，BF19 表现出 53.33% 的生防效，并显著提高了平均株高、根鲜重和干重。全基因组测序和比较基因组分析揭示了 BF19 中许多与生物控制机制相关的潜在基因。重要的是，代谢簇预测与液相色谱-串联质谱（LC\u2012MS/MS）的整合揭示了 MACROBREVIN 抗生素的存在，这是一种聚酮合酶（PKS）的产物，主要存在于 BF19 发酵产物中。Br. brevis 菌株 BF19 及其粗提取物对苦瓜枯萎病的有效性也已得到证实。本研究为未来 PKS 的研究提供了遗传框架，并为优化源自短 Br. brevis BF19 的微生物生物农药的田间应用建立了科学基础。

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Comprehensive genomic analysis of Brevibacillus brevis BF19 reveals its biocontrol potential against bitter gourd wilt

Bitter gourd wilt, a severe vascular disease triggered by the soilborne pathogen Fusarium oxysporum f. sp. momordicae (FOM), markedly constrains bitter gourd yield. In this study, a novel strain BF19 of Brevibacillus brevis was isolated and identified, exhibiting strong antimicrobial activity against FOM through in vivo and in vitro experiments. To comprehensively assess the biocontrol potential of strain BF19, we conducted phenotypic, phylogenetic, and comparative genomics analyses. Phenotypic analysis revealed that BF19 exhibited 53.33% biocontrol efficacy and significantly increased the average plant height, root fresh weight, and dry weight. Whole-genome sequencing and comparative genomic analysis revealed numerous potential genes associated with biocontrol mechanisms in BF19. Importantly, the integration of metabolic cluster prediction with liquid chromatography‒tandem mass spectrometry (LC‒MS/MS) revealed the presence of a macrobrevin antibiotic, a product of polyketide synthases (PKSs), predominantly in BF19 fermentation products. The effectiveness of the Br. brevis strain BF19 and its crude extract against bitter gourd wilt has also been confirmed. This study provides a genetic framework for future investigations on PKSs and establishes a scientific basis for optimizing field applications of microbial biopesticides derived from Br. brevis BF19.

更新日期：2024-10-18

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