This work evaluated the extraction, purification, and enrichment process of peony seed shell (PSS) polysaccharides, and explored the structural characteristics and the antioxidant activity of the polysaccharides. In this study, water was used as the solvent in combination with ultrasonic-assisted extraction technology to extract polysaccharides from PSS, and the extraction process was refined and optimized. The optimal extraction conditions were obtained as follows: liquid-to-solid ratio of 15 mL/g, power ratio of 65 %, extraction time of 25 min, and extraction temperature of 44°C. The obtained polysaccharide solution was precipitated with 80 % ethanol and 100 % ethanol to obtain polysaccharides PP-a and PP-b, with yields of 1.39 % and 1.11 %, respectively. The crude polysaccharide was removed from protein by enzymatic method, followed by further separation and purification using DEAE-52 and Sephadex G-100 column chromatography. Two polysaccharide components, PP-a-3 and PP-b-1, were successfully obtained. The structures of the two polysaccharides were identified using methods such as Fourier-Transform Infrared Spectroscopy (FT-IR), monosaccharide composition analysis, methylation analysis, and nuclear magnetic resonance (NMR) spectrum. The results implied that PP-a-3 was mainly composed of (1→4,5)-arabinose and (1→2,4,5)-galactose, while PP-b-1 was mainly composed of (1→4,5)-arabinose. The results of in vitro antioxidant activity suggested that PP-a-3 and PP-b-1 exhibited enhanced antioxidant capacity with increasing concentration in the range of 1–8 mg/mL. The results revealed that the two polysaccharides had good antioxidant properties and could be used as natural antioxidants. This study laid the research foundation for the deep development and utilization of oil tree peony, and also provided the reference for the further study and development of active ingredients in natural products.

中文翻译：

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牡丹籽壳多糖的提取、分离及抗氧化活性


本工作评价了牡丹籽壳 （PSS） 多糖的提取、纯化和富集过程，并探讨了多糖的结构特性和抗氧化活性。本研究以水为溶剂，结合超声辅助提取技术提取 PSS 多糖，并对提取工艺进行细化和优化。最佳提取条件为：液固比 15 mL/g，功率比 65 %，提取时间 25 min，提取温度 44°C。 将所得多糖溶液用 80 % 乙醇和 100 % 乙醇沉淀，得到多糖 PP-a 和 PP-b，收率分别为 1.39 % 和 1.11 %。通过酶法从蛋白质中去除粗多糖，然后使用 DEAE-52 和 Sephadex G-100 柱层析进一步分离和纯化。成功获得 PP-a-3 和 PP-b-1 两种多糖组分。使用傅里叶变换红外光谱 （FT-IR）、单糖组成分析、甲基化分析和核磁共振 （NMR） 光谱等方法鉴定两种多糖的结构。结果表明，PP-a-3 主要由 （1→4,5）-阿拉伯糖和 （1→2,4,5）-半乳糖组成，而 PP-b-1 主要由 （1→4,5）-阿拉伯糖组成。体外抗氧化活性结果表明，PP-a-3 和 PP-b-1 在 1-8 mg/mL 的浓度范围内随着浓度的增加而增强。结果表明，这两种多糖具有良好的抗氧化性能，可作为天然抗氧化剂。 本研究为油树牡丹的深度开发利用奠定了研究基础，也为天然产物中活性成分的进一步研究和开发提供了参考。