In this study, we investigated the effects of transplanting Ningxiang pig fecal bacteria on ileum microflora and intestinal barrier of Duroc × Landrace × Large White (DLY) pigs. Thirty-two DLY pigs at 90-d-old were equally assigned to either control groups (fed the basal diet) or test group (fed the basal diet + 10ml fecal microbiota suspension from Ningxiang pig). Results showed that fecal microbiota transplantation (FMT) did not influence the growth performance, but increased the number of ileum goblet cells and the expression level of mucin-2. Additionally, the mucosal levels of anti-inflammatory cytokines interlukin-4 and interlukin-10 were upregulated, but the level of pro-inflammatory cytokine interferon-γ was downregulated by FMT. Moreover, FMT increased the expression level of porcine β defensin-114 in ileum mucus. 16S rRNA gene sequencing of ileal digesta showed that FMT modulated the diversity and composition of ileal microbiota of DLY pigs by increasing the relative abundances of beneficial bacteria, while decreasing the abundance of the pathogenic bacterium Streptococcus. Taken together, the study showed that FMT of Ningxiang pigs could improve intestinal barrier condition of DLY pigs by improving intestinal microflora and promoting intestinal health.

中文翻译：

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不同品种粪便微生物群移植改善了受体猪的肠道屏障状况并调节了回肠菌群


本研究探讨了宁乡猪粪便细菌移植对杜洛克×长白猪×大白猪回肠菌群和肠道屏障的影响。将 32 头 90 d 龄 DLY 猪平均分配到对照组 （饲喂基础日粮） 或试验组 （饲喂宁乡猪基础日粮 + 10 ml 粪便微生物群悬浮液）。结果显示，粪便微生物群移植 （FMT） 不影响生长性能，但增加了回肠杯状细胞的数量和粘蛋白-2 的表达水平。此外，FMT 上调了抗炎细胞因子 interlukin-4 和 interlukin-10 的粘膜水平，但促炎细胞因子干扰素-γ 的水平被 FMT 下调。此外，FMT 增加了回肠粘液中猪 β 防御素-114 的表达水平。回肠消化 16S rRNA 基因测序显示，FMT 通过增加有益菌的相对丰度，同时降低病原菌链球菌的丰度，调节 DLY 猪回肠微生物群的多样性和组成。综上所述，宁乡猪 FMT 可通过改善肠道菌群和促进肠道健康来改善 DLY 猪的肠道屏障状况。