A study in Scientific Reports has used isotransplantation to examine the effect of mouse breeding nuclei in prostate cancer development and intratumoural macrophage populations, illustrating the importance of understanding mouse models to explore the tumour immune microenviroment (TIME). TRAMP-C1 cells were implanted into C57BL/6J mice from two breeding nuclei (nA and nB) and tumour growth period and intratumoural macrophage profile were measured. After 69 days, 54% of nB mice showed tumour implantation, whereas 100% of nA mice showed implantation after 28 days. A significantly increased M2 macrophage profile was observed in the TIME from both mice groups, but they were not significantly different from each other. However, nB tumours exhibited approximately twice the population of M1 macrophages (11–27%) of nA (4–15%) and fewer non-polarized macrophages; M1:M2 average ratio was 1:8 for group nA and 1:4 for nB. These differing tumour progression and macrophage populations in mice from the same substrain illustrate the importance of animal source renewal for controlling murine cancer model variables, especially in TIME research.

Scientific Reports 上的一项研究使用异移植来检查小鼠繁殖核对前列腺癌发展和瘤内巨噬细胞群的影响，说明了了解小鼠模型对探索肿瘤免疫微环境 （TIME） 的重要性。将 TRAMP-C1 细胞从两个繁殖核 （nA 和 nB） 植入 C57BL/6J 小鼠体内，测量肿瘤生长期和瘤内巨噬细胞谱。69 天后，54% 的 nB 小鼠出现肿瘤植入，而 100% 的 nA 小鼠在 28 天后出现植入。在时间中观察到两组小鼠的 M2 巨噬细胞谱显著增加，但它们彼此之间没有显著差异。然而，nB 肿瘤表现出大约 m1 巨噬细胞数量 （11-27%） 的两倍 nA （4-15%） 和非极化巨噬细胞较少;nA 组的 M1：M2 平均比值为 1：8，nB 组为 1：4。来自同一亚系的小鼠的这些不同的肿瘤进展和巨噬细胞群说明了动物来源更新对于控制小鼠癌症模型变量的重要性，尤其是在 TIME 研究中。