The misplacement of proteins within a cell can cause various diseases, but no therapeutic intervention can precisely control protein location. Writing in Nature, Ng et al. describe targeted relocalization-activating molecules (TRAMs) that couple misplaced proteins to cellular shuttles, relocating them to their proper places.

Ng et al. began by testing a TRAM previously used to bring together two proteins, EcDHFR and FKBP12^F36V. They converted EcDHFR into a shuttle protein by adding a nuclear export signal and coupled FKBP12^F36V to a target protein in the nucleus, enabling its movement to the cytoplasm upon TRAM treatment.

蛋白质在细胞内的错位会导致各种疾病，但没有治疗干预可以精确控制蛋白质的位置。Ng 等人在《自然》杂志上撰文，描述了靶向再定位激活分子 （TRAM），这些分子将错位的蛋白质偶联到细胞穿梭机上，将它们重新定位到适当的位置。

Ng 等人首先测试了以前用于将两种蛋白质 EcDHFR 和 FKBP12^F36V 结合在一起的 TRAM。他们通过添加核输出信号并将 FKBP12^F36V 偶联到细胞核中的靶蛋白，将 EcDHFR 转化为穿梭蛋白，使其能够在 TRAM 处理后移动到细胞质。