This work presents an innovative all-electrical platform for selective single-particle manipulation. The platform combines microfluidic impedance cytometry for label-free particle characterization and dielectrophoresis for contactless multi-way particle separation. The microfluidic chip has a straightforward coplanar electrode layout and no particle pre-focusing mechanism is required. An original online algorithm analyzes the impedance signals of each incoming particle and regulates in real time the dielectrophoretic voltages according to a desired control logic. As a proof-of-concept, three operation modes are demonstrated on a mixture of 8, 10, and 12 μm diameter beads: (i) particle position swapping across the channel axis, irrespective of particle size, (ii) size-based particle separation, irrespective of particle position, and (iii) sorting of a selected sequence of particles. As a perspective, the versatility of impedance cytometry and dielectrophoresis, and the possibility of configuring alternative control logics, hold promise for advanced particle and cell manipulation.

中文翻译：

---

实时阻抗激活介电泳驱动，用于可重新配置单个流动颗粒的操作


这项工作提出了一种用于选择性单粒子操作的创新全电平台。该平台结合了微流体阻抗细胞术和介电泳技术，用于无标记颗粒表征，以及用于非接触式多路颗粒分离。微流控芯片具有简单的共面电极布局，不需要粒子预聚焦机制。原始的在线算法分析每个输入粒子的阻抗信号，并根据所需的控制逻辑实时调节介电泳电压。作为概念验证，在直径为 8、10 和 12 μm 的微珠混合物上演示了三种操作模式：（i） 跨通道轴的颗粒位置交换，与颗粒大小无关，（ii） 基于大小的颗粒分离，与颗粒位置无关，以及 （iii） 对选定的颗粒序列进行排序。从某种角度来看，阻抗细胞术和介电泳的多功能性，以及配置替代控制逻辑的可能性，为先进的颗粒和细胞操作带来了希望。