The egg parasitoids, Trichogramma evanescens Westwood and T. pinto Voegelé (Hymenoptera: Trichogrammatidae), play central roles in the management of lepidopteran crop pests. However, their effectiveness highly depends on the employment of compatible pesticides, with a particular focus on novel insecticides that are promising in pest control. This study investigated the lethal, sublethal and transgenerational effects of two novel insecticides, afidopyropen and broflanilide, on T. evanescens and T. pintoi in terms of biological and behavioral traits. The eggs of the factitious host, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae), with parasitoids at the preimaginal developmental stages (egg-larval, prepupal and pupal) were immersed in insecticide solutions. The emergence rates of both parasitoids were negatively affected by preimaginal treatments with different concentrations of afidopyropen and broflanilide. In transgenerational experiments, broflanilide treatments significantly reduced the parasitism performance of the treated generation (F0), emergence rate of F1 and F2, sex ratio of F1 T. evanescens and the emergence rate and sex ratio of F1 T. pintoi. Afidopyropen treatments reduced the emergence rate of F3 T. evanescens adults. The longevity of the F0 females and males of both parasitoids and that of the F1 females of T. evanescens were significantly reduced by broflanilide treatments. Broflanilide also decreased the walking speed of both species while afidopyropen enhanced the resting frequency of T. evanescens. This comprehensive study provides insights into the toxicological assessment of afidopyropen and broflanilide on Trichogramma species, employing parameters corresponding to their population and behavioral dynamics. Finally, this study concludes that the integration of afidopyropen and broflanilide into integrated pest management (IPM) strategies necessitates careful consideration, particularly regarding potential non-target effects on Trichogramma species.

卵寄生虫 Trichogramma evanescens Westwood 和 T. pinto Voegelé（膜翅目：Trichogrammatidae）在鳞翅目作物害虫的管理中起着核心作用。然而，它们的有效性在很大程度上取决于使用相容性杀虫剂，特别关注在害虫控制方面有希望的新型杀虫剂。本研究调查了两种新型杀虫剂 afidopyropen 和 broflanilide 在生物学和行为特性方面对 T. evanescens 和 T. pintoi 的致死、亚致死和跨代影响。将人造宿主 Ephestia kuehniella Zeller （鳞翅目：Pyralidae） 的卵与处于想象前发育阶段的寄生虫（卵幼虫、前蛹和蛹）浸泡在杀虫剂溶液中。两种寄生虫的出现率都受到不同浓度的 afidopyropen 和 broflanilide 的想象前处理的负面影响。在跨代实验中，溴羊毛酰化物处理显著降低了处理一代的寄生性能 （F0） 、 F1 和 F2 的出苗率、 F1 T. evanescens 的性别比以及 F1 T. pintoi 的出苗率和性别比。Afidopyropen 处理降低了 F3 T. evanescens 成虫的出现率。溴羊毛酰化物处理显著降低了两种寄生虫的 F0 雌性和雄性以及 T. evanescens 的 F1 雌性的寿命。溴苯酰胺还降低了这两个物种的行走速度，而 afidopyropen 提高了 T. evanescens 的静息频率。 这项全面的研究提供了对 afidopyropen 和 broflanilide 对 Trichogramma 物种的毒理学评估的见解，采用与其种群和行为动力学相对应的参数。最后，本研究得出结论，将 afidopyropen 和 broflanilide 整合到综合害虫管理 （IPM） 策略中需要仔细考虑，特别是关于对毛滴虫物种的潜在非靶向影响。