Industrial hemp is a valuable fibrous plant with high biomass production for it can survive under variable environmental stresses including heavy metals and is used in a wide range of products. This research work aimed at determining the growth potential of industrial hemp (Cannabis sativa) developed on lead (Pb) contaminated mine soils using various chelating agents viz., ethylenediaminedisuccinic acid (EDDS), ethylenediaminetetraacetic acid (EDTA), citric acid (CA) and nitrilotriacetic acid (NTA) under various concentrations of nitrogen (N) fertilizer such as 0 (control), 50, 150, 250 and 350 kg ha⁻¹. Results indicated that the synergistic effect of chelating agents and rising concentrations of N fertilizer resulted in a linear increase in hemp growth and dry biomass. The chelating agent and N mediated showed better response in the photosynthetic, antioxidative, and osmolytic potential of hemp as compared with control. The concentration of N and Pb plants showed a differential response such as N accumulation was maximum in plant leaf and shoot portions as compared to the roots. In contrast, Pb enrichment was higher in the roots than in the plant’s leaves and stems when exposed to varying concentrations of N fertilizer and chelating agents in the soil environment. Biomobilizaiton of Pb in the plant continuum enhanced with the rising N fertilizer levels in the soil. The EDTA showed better phytoextraction potential as compared with the rest of the chelating agents. Hence, findings suggest that the synergistic application of chelating agents and N fertilizer is a far more effective technique that increases the bioavailability of Pb for phytoextraction and decreases Pb-induced oxidative injuries by restricting Pb uptake.

中文翻译：

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麻中螯合剂与氮的协同作用对铅污染矿土的植物修复


工业大麻是一种有价值的纤维植物，具有高生物量产量，因为它可以在包括重金属在内的各种环境压力下生存，并被广泛用于各种产品中。本研究工作旨在确定使用各种螯合剂，即乙二胺二琥珀酸 （EDDS）、乙二胺四乙酸 （EDTA）、柠檬酸 （CA） 和次氮基三乙酸 （NTA） 在各种浓度的氮 （N） 肥料（如 0（对照）、50、150、250 和 350 kg ^ha-1 下，在铅 （Pb） 污染的矿山土壤上开发的工业大麻（大麻）的生长潜力-1.结果表明，螯合剂和氮肥浓度升高的协同作用导致大麻生长和干生物量线性增加。与对照相比，螯合剂和 N 介导的大麻的光合作用、抗氧化和渗透电位反应更好。N 和 Pb 植物的浓度表现出差异响应，例如与根相比，植物叶片和芽部分的 N 积累最大。相比之下，当土壤环境中暴露于不同浓度的氮肥和螯合剂时，根中的 Pb 富集高于植物的叶和茎。植物连续体中 Pb 的生物移动性随着土壤中氮肥水平的升高而增强。与其他螯合剂相比，EDTA 显示出更好的植物提取潜力。因此，研究结果表明，螯合剂和氮肥的协同应用是一种更有效的技术，它通过限制 Pb 的吸收来提高植物提取中 Pb 的生物利用度并减少 Pb 诱导的氧化损伤。