The industrial preparation of kudzu starch (KS) significantly reduces the remaining of flavonoids like puerarin (PU) in the product, weakening its biological activity and making pre-treatments on kudzu crucial. Ultrasonic technique, widely used for modifying biomolecules, can enhance nutrient interactions like those between starch and polyphenols in foods. Thus, a puerarin-kudzu starch (PKS) complex was prepared with the introduction of ultrasonic pretreatment. The results indicated that sonication increased the binding of PU to KS from 0.399 ± 0.01 to 0.609 ± 0.05 mg/g. Particle size analysis and SEM revealed that the particles of the ultrasonic puerarin-kudzu starch complex (UPKS) were larger than those of the untreated complexes. XRD, UV–vis, and FT-IR spectroscopic analyses indicated that hydrogen bonding primarily governs the interaction between PU and KS. Additionally, incorporating PU decreased the starch structure’s orderliness, while ultrasonic treatment altered the helical configuration of straight-chain starch, leading to the formation of a new, ordered structure through the creation of new hydrogen bonds. Additionally, gels formed from UPKS exhibited higher viscosity, elasticity, and shear stress, suggesting that ultrasound significantly altered the intermolecular interactions between PKS. In conclusion, the use of ultrasound under optimal conditions has demonstrated its effectiveness in preparing PKS complexes, highlighting its significant potential to produce high value-added kudzu-based products.

中文翻译：

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通过各种超声预处理改善葛根素与葛根淀粉的络合：相互作用机制分析


葛根淀粉 （KS） 的工业制备显着减少了产品中葛根素 （PU） 等黄酮类化合物的残留，削弱了其生物活性，使葛根的预处理变得至关重要。超声波技术广泛用于修饰生物分子，可以增强营养物质的相互作用，例如食品中淀粉和多酚之间的相互作用。因此，通过引入超声预处理制备了葛根-葛根淀粉 （PKS） 复合物。结果表明，超声处理将 PU 与 KS 的结合从 0.399 ± 0.01 增加到 0.609 ± 0.05 mg/g。粒度分析和 SEM 显示，超声葛根-葛根淀粉复合物 （UPKS） 的颗粒比未处理的复合物的颗粒大。XRD、UV-Vis 和 FT-IR 光谱分析表明，氢键主要控制 PU 和 KS 之间的相互作用。此外，掺入 PU 降低了淀粉结构的有序性，而超声处理改变了直链淀粉的螺旋构型，通过创建新的氢键形成了新的有序结构。此外，由 UPKS 形成的凝胶表现出更高的粘度、弹性和剪切应力，这表明超声显着改变了 PKS 之间的分子间相互作用。总之，在最佳条件下使用超声证明了其在制备 PKS 复合物方面的有效性，突出了其生产高附加值葛根产品的巨大潜力。