Enterotoxigenic Escherichia coli (E. coli) is a threat to humans and animals that causes intestinal disorders. Antimicrobial resistance has urged alternatives, including Lactobacillus postbiotics, to mitigate the effects of enterotoxigenic E. coli. Forty-eight newly weaned pigs were allotted to NC: no challenge/no supplement; PC: F18+ E. coli challenge/no supplement; ATB: F18+ E. coli challenge/bacitracin; and LPB: F18+ E. coli challenge/postbiotics and fed diets for 28 d. On d 7, pigs were orally inoculated with F18+ E. coli. At d 28, the mucosa-associated microbiota, immune and oxidative stress status, intestinal morphology, the gene expression of pattern recognition receptors (PRR), and intestinal barrier function were measured. Data were analyzed using the MIXED procedure in SAS 9.4. PC increased (P < 0.05) Helicobacter mastomyrinus whereas reduced (P < 0.05) Prevotella copri and P. stercorea compared to NC. The LPB increased (P < 0.05) P. stercorea and Dialister succinatiphilus compared with PC. The ATB increased (P < 0.05) Propionibacterium acnes, Corynebacterium glutamicum, and Sphingomonas pseudosanguinis compared to PC. The PC tended to reduce (P = 0.054) PGLYRP4 and increased (P < 0.05) TLR4, CD14, MDA, and crypt cell proliferation compared with NC. The ATB reduced (P < 0.05) NOD1 compared with PC. The LPB increased (P < 0.05) PGLYRP4, and interferon-γ and reduced (P < 0.05) NOD1 compared with PC. The ATB and LPB reduced (P < 0.05) TNF-α and MDA compared with PC. The F18+ E. coli challenge compromised intestinal health. Bacitracin increased beneficial bacteria showing a trend towards increasing the intestinal barrier function, possibly by reducing the expression of PRR genes. Lactobacillus postbiotics enhanced the immunocompetence of nursery pigs by increasing the expression of interferon-γ and PGLYRP4, and by reducing TLR4, NOD1, and CD14.

中文翻译：

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日粮乳酸菌后生元和杆菌肽对产肠毒素 F18+ 大肠埃希菌攻击猪黏膜相关菌群和影响空肠粘膜免疫能力的模式识别受体调节的影响


产肠毒素大肠杆菌 （E. coli） 对人类和动物构成威胁，可导致肠道疾病。抗菌素耐药性敦促包括乳酸菌后生元在内的替代品来减轻产肠毒素大肠杆菌的影响。48 头新断奶仔猪被分配到 NC：无激发/无添加剂;PC：F18+ 大肠杆菌攻击/无添加剂;ATB：F18+ 大肠杆菌攻击/杆菌肽;和 LPB：F18 + 大肠杆菌攻击/后生元和进食饮食 28 天。第 7 天，猪经口接种 F18 + 大肠杆菌。在 28 天时，测量粘膜相关微生物群、免疫和氧化应激状态、肠道形态、模式识别受体 （PRR） 基因表达和肠道屏障功能。使用 SAS 9.4 中的 MIXED 程序分析数据。与 NC 相比，PC 增加 （P < 0.05） 乳胶霉菌，而减少 （P < 0.05） Prevotella copri 和 P. stercorea。与 PC 相比，LPB 增加 （P < 0.05） P. stercorea 和 Dialister succinatiphilus。与 PC 相比，ATB 增加 （P < 0.05） 痤疮丙酸杆菌、谷氨酸棒状杆菌和假血鞘氨醇单胞菌。与 NC 相比，PC 倾向于降低 （P = 0.054） PGLYRP4并增加 （P < 0.05） TLR4 、 CD14 、 MDA 和隐窝细胞增殖。与 PC 相比，ATB 降低 （P < 0.05） NOD1。与 PC 相比，LPB 增加 （P < 0.05） PGLYRP4，干扰素γ和降低 （P < 0.05） NOD1。与 PC 相比，ATB 和 LPB 降低了 （P < 0.05） TNF-α 和 MDA。F18+ 大肠杆菌攻击损害了肠道健康。杆菌肽增加有益菌，显示出增加肠道屏障功能的趋势，可能是通过减少 PRR 基因的表达。 乳酸菌后生元通过增加干扰素γ和PGLYRP4的表达，以及通过降低 TLR4 、 NOD1 和 CD14 来增强保育猪的免疫能力。