RNA polymerases initiate along a genome bound by nucleosomes, transcription factors, chromatin remodellers and other RNA polymerase complexes. Understanding the interplay between transcription and chromatin landscapes has been limited by correlative comparisons.

Tullius et al. used a high-resolution, long-read, single-molecule fibre sequencing method — Fiber-seq — to study the competition and co-ordination between RNA polymerases and the surrounding nucleosomes along individual chromatin fibres.

中文翻译：

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可视化 RNA 聚合酶动力学

RNA 聚合酶沿着与核小体、转录因子、染色质重塑剂和其他 RNA 聚合酶复合物结合的基因组启动。了解转录和染色质景观之间的相互作用受到相关比较的限制。

Tullius 等人使用高分辨率、长读长、单分子纤维测序方法 Fiber-seq 来研究 RNA 聚合酶与周围核小体之间沿单个染色质纤维的竞争和协调。