Water microbial contamination is a serious issue that poses a risk to both animal and human health. One hundred and twenty water were samples collected from main water source and drinkers from a poultry farm. Different bacterial pathogens were isolated from water sources. Escherichia (E.) coli, Pseudomonas (P.) aureoginosa, (Salmonella) Salmonella (s). Typhimurim, Aeromonas (A.) hydrophila at different percentages. Variable degree of bacterial resistance to some commercial disinfectants commonly used to disinfect water system (iodine, terminator and H₂O₂). Nanoparticles were used to control bacteria in water. About the safety investigation for the prepared nanomaterials, the work results demonstrated that zinc oxide (ZnO) nanoparticles (NPs) exhibit the highest safety profile among the manufactured materials. The median fatal dose (LD₅₀) for ZnO NPs was determined to be 3709 mg/kg body weight. In comparison, the LD₅₀ values for zeolites and nanocomposites were 3251 mg/kg and 2658 mg/kg, respectively. Therapeutic dosages were estimated based on the LD₅₀. Zeolite NPs, ZnO NPs and ZnO/zeolite NPs showing promising results in control of those bacteria. It was concluded that the escalating resistance of bacteria to disinfectants have led to a need to find alternative such as nanoparticles that proved promising results in control of pathogens, particularly it showed a safe effect on laboratory animals.

水微生物污染是一个严重的问题，对动物和人类健康构成风险。120 份水是从家禽养殖场的主要水源和饮水器收集的样本。从水源中分离出不同的细菌病原体。埃希氏菌 （E.） 大肠杆菌、假单胞菌 （P.） aureoginosa，（沙门氏菌）沙门氏菌 （s）。鼠伤寒， 气单胞菌 （A.）不同百分比的嗜水性。对一些常用于水系统消毒的商用消毒剂（碘、终结剂和 H₂O₂）具有不同程度的细菌耐药性。纳米颗粒用于控制水中的细菌。关于所制备纳米材料的安全性调查，工作结果表明，氧化锌 （ZnO） 纳米颗粒 （NPs） 在制造材料中表现出最高的安全性。ZnO NPs 的中位致死剂量 （LD₅₀） 确定为 3709 mg/kg 体重。相比之下，沸石和纳米复合材料的 LD₅₀ 值分别为 3251 mg/kg 和 2658 mg/kg。根据 LD₅₀ 估计治疗剂量。沸石 NPs、ZnO NPs 和 ZnO/沸石 NPs 在控制这些细菌方面显示出有希望的结果。得出的结论是，细菌对消毒剂的耐药性不断升级，导致需要寻找替代品，例如纳米颗粒，这些替代品在控制病原体方面被证明是有希望的结果，特别是它对实验动物显示出安全效果。