<br>蚕丝素蛋白和蚕丝素蛋白/纳米羟基磷灰石支架在组织再生应用中的体外巨噬细胞反应研究,ACS Biomaterials Science & Engineering

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蚕丝素蛋白和蚕丝素蛋白/纳米羟基磷灰石支架在组织再生应用中的体外巨噬细胞反应研究
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2024-10-09 , DOI: 10.1021/acsbiomaterials.4c00976
Kallista Wong ₁ , Xuan Hao Tan ₁ , Jun Li ₁ , James Hoi Po Hui ₁ , James Cho Hong Goh ₁

Affiliation

近年来，由于 SF 的机械强度和 nHA 的骨传导特性，丝素蛋白（SF）已与低结晶度纳米羟基磷灰石（nHA）一起作为各种组织再生应用的支架。然而，目前对植入后 SF 与 nHA 复合材料降解产物的免疫反应缺乏了解。众所周知，生物材料降解产生的颗粒碎片可以触发免疫反应。由于支架由可降解材料制成，降解产物可能会导致炎症。因此，在本研究中，与对照 SF 支架相比，研究了 SF/nHA 支架的酶降解对巨噬细胞反应的影响。由于支架的降解产物可以影响巨噬细胞极化，因此可以假设，由于 SF 和 SF/nHA 支架在使用蛋白酶 XIV 溶液在体外降解，降解产物有助于 THP-1 衍生的巨噬细胞从促炎 M1 极化为抗炎 M2 表型。结果表明，SF/nHA 支架的初始（第 1 天）降解产物引发了促炎反应，而 SF/nHA 支架的后（第 24 天）降解产物引发了抗炎反应。此外，由于 SF 支架的降解速度更快且降解溶液中的氨基酸浓度较高，SF 支架的降解产物引起了更高的抗炎反应。因此，本文可以帮助阐明 SF 和 SF/nHA 支架降解产物对巨噬细胞反应的贡献影响，并为设计具有可调降解速率的丝基生物材料提供更深入的见解，这些材料可以调节巨噬细胞反应，用于未来的组织再生应用。

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An In Vitro Macrophage Response Study of Silk Fibroin and Silk Fibroin/Nano-Hydroxyapatite Scaffolds for Tissue Regeneration Application

In recent years, silk fibroin (SF) has been incorporated with low crystallinity nanohydroxyapatite (nHA) as a scaffold for various tissue regeneration applications due to the mechanical strength of SF and osteoconductive properties of nHA. However, currently, there is a lack of understanding of the immune response toward the degradation products of SF with nHA composite after implantation. It is known that particulate fragments from the degradation of a biomaterial can trigger an immune response. As the scaffold is made of degradable materials, the degradation products may contribute to the inflammation. Therefore, in this study, the effects of the enzymatic degradation of the SF/nHA scaffold on macrophage response were investigated in comparison to the control SF scaffold. Since the degradation products of a scaffold can influence macrophage polarization, it can be hypothesized that as the SF and SF/nHA scaffolds were degraded in vitro using protease XIV solution, the degradation products can contribute to the polarization of THP-1-derived macrophages from pro-inflammatory M1 to anti-inflammatory M2 phenotype. The results demonstrated that the initial (day 1) degradation products of the SF/nHA scaffold elicited a pro-inflammatory response, while the latter (day 24) degradation products of the SF/nHA scaffold elicited an anti-inflammatory response. Moreover, the degradation products from the SF scaffold elicited a higher anti-inflammatory response due to the faster degradation of the SF scaffold and a higher amino acid concentration in the degradation solution. Hence, this paper can help elucidate the contributory effects of the degradation products of SF and SF/nHA scaffolds on macrophage response and provide greater insights into designing silk-based biomaterials with tunable degradation rates that can modulate macrophage response for future tissue regeneration applications.

更新日期：2024-10-09

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