Osteomyelitis occurs when Staphylococcus aureus invades the bone microenvironment, resulting in a bone marrow abscess with a spatially defined architecture of cells and biomolecules. Imaging mass spectrometry and microscopy are tools that can be employed to interrogate the lipidome of S. aureus-infected murine femurs and reveal metabolic and signaling consequences of infection. Here, nearly 250 lipids were spatially mapped to healthy and infection-associated morphological features throughout the femur, establishing composition profiles for tissue types. Ether lipids and arachidonoyl lipids were altered between cells and tissue structures in abscesses, suggesting their roles in abscess formation and inflammatory signaling. Sterols, triglycerides, bis(monoacylglycero)phosphates, and gangliosides possessed ring-like distributions throughout the abscess, suggesting a hypothesized dysregulation of lipid metabolism in a population of cells that cannot be discerned with traditional microscopy. These data provide insight into the signaling function and metabolism of cells in the fibrotic border of abscesses, likely characteristic of lipid-laden macrophages.

中文翻译：

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使用多模态成像质谱法揭示金黄色葡萄球菌骨髓炎中的脂质动力学


当金黄色葡萄球菌侵入骨微环境时，就会发生骨髓炎，导致骨髓脓肿具有空间定义的细胞和生物分子结构。成像、质谱和显微镜是可用于检测金黄色葡萄球菌 感染的小鼠股骨脂质组并揭示感染的代谢和信号传导后果的工具。在这里，近 250 种脂质在空间上映射到整个股骨的健康和感染相关形态特征，从而建立了组织类型的组成图谱。醚脂质和花生四烯酰脂质在脓肿的细胞和组织结构之间发生改变，表明它们在脓肿形成和炎症信号传导中的作用。甾醇、甘油三酯、双（单酰基甘油）磷酸酯和神经节苷脂在整个脓肿中呈环状分布，表明假设的传统显微镜无法识别的细胞群中脂质代谢失调。这些数据提供了对脓肿纤维化边界细胞信号传导功能和代谢的见解，这可能是富含脂质的巨噬细胞的特征。