The papaya mealybug, Paracoccus marginatus, is an invasive pest that causes significant damage to various tropical fruits and ornamentals worldwide. Lambda-cyhalothrin, an effective insecticide in integrated pest management (IPM) strategies, can control P. marginatus. This study evaluated the sublethal effect of lambda-cyhalothrin on the life table parameters and the detoxification enzymes activities of P. marginatus. Furthermore, transcriptome sequencing analysis revealed the potential mechanisms of the detoxification-related genes associated with enzymes activities. The study reported that lambda-cyhalothrin showed high activity against P. marginatus, with LC₃₀ values of 8.98 mg/L. Two-sex life table results showed that the development duration of the second instar nymphs, preadults, and adult female longevity was significantly prolonged, and the fecundity was stimulated in the F₁-CY of P. marginatus when the parental generation was exposed to LC₃₀ lambda-cyhalothrin. Moreover, lambda-cyhalothrin treatment significantly increased the activities of Cytochrome P450 monooxygenases (P450) and glutathione-S-transferase (GST) enzymes. Additionally, significant expression levels of P450, GST, carboxylesterase, and esterase genes were observed in P. marginatus subjected to the sublethal lambda-cyhalothrin exposure. Our findings demonstrate that sublethal concentrations of lambda-cyhalothrin influenced the development and fecundity of P. marginatus, and changes in enzymes activities and differential expression of detoxifying genes might be involved in the response and detoxification metabolism of lambda-cyhalothrin in P. marginatus. These results could contribute to a comprehensive risk assessment and provide guidance for the scientific use of lambda-cyhalothrin in IPM strategies.

木瓜粉蚧 Paracoccus marginatus 是一种入侵性害虫，对世界各地的各种热带水果和观赏植物造成重大破坏。Lambda-cyhalothrin 是综合害虫管理 （IPM） 策略中的一种有效杀虫剂，可以控制 P. marginatus。本研究评价了 lambda-cyhalothrin 对 P. marginatus 生命表参数和解毒酶活性的亚致死影响。此外，转录组测序分析揭示了与酶活性相关的解毒相关基因的潜在机制。研究报告称，lambda-cyhalothrin 对 P. marginatus 表现出高活性，LC₃₀ 值为 8.98 mg/L。两性生命表结果显示，当亲代暴露于 LC 时，第二龄若虫、前成体和成年雌性寿命的发育持续时间显著延长，并且 P. marginatus 的 F_1-CY 的繁殖力受到刺激₃₀lambda-氯氟氰菊酯。此外，lambda-cyhalothrin 处理显著提高了细胞色素 P450 单加氧酶 （P450） 和谷胱甘肽-S-转移酶 （GST） 酶的活性。此外，在亚致死性 lambda-cyhalothrin 暴露的 P. marginatus 中观察到 P450 、 GST 、羧酸酯酶和酯酶基因的显著表达水平。我们的研究结果表明，亚致死浓度的 lambda-cyhalothrin 影响了 P. marginatus 的发育和繁殖力，酶活性的变化和解毒基因的差异表达可能参与了 P. marginatus 中 lambda-氯氟氰菊酯的反应和解毒代谢。这些结果有助于全面的风险评估，并为 lambda-cyhalothrin 在 IPM 策略中的科学使用提供指导。