Background:Injuries to the glenoid labrum have been recognized as a source of joint pain and discomfort, which may be associated with the inflammatory responses that lead to the deterioration of labral tissue. However, it is unclear whether the torn labrum prompts mast cell (MC) activation, resulting in synovial inflammatory responses that lead to labral tissue degeneration.Purpose:To determine the potential influence of activated MC on synovial inflammatory responses and subsequent labral tissue degeneration and shoulder function deterioration in a rat model by monitoring MC behavior and sequential inflammatory responses within the synovial tissue and labral tissue after injury, suture repair, and MC stabilizer administration.Study Design:Controlled laboratory study.Methods:Anteroinferior glenoid labral tears were generated in the right shoulder of rats (n = 20) and repaired using a tunneled suture technique. Synovial tissue inflammatory responses were modulated in some rats with intraperitoneal administration of an MC stabilizer—cromolyn (n = 10). At weeks 1 and 3, MC activation, synovial inflammatory responses, and labral degeneration were histologically evaluated. Simultaneously, gait analysis was performed before and after surgical repair to assess the worsening of the shoulder function after the injury and treatment.Results:Resident MC degranulation after labral injury (50.48% ± 8.23% activated at week 1) contributed to the initiation of synovial tissue inflammatory cell recruitment, inflammatory product release, matrix metalloproteinase-13, and subsequent labral tissue extracellular matrix degeneration. The administration of cromolyn, an MC stabilizer, was found to significantly diminish injury-mediated inflammatory responses (inflammatory cell infiltration and subsequent proinflammatory product secretion) and improve shoulder functional recovery.Conclusion:MC activation is responsible for labral tear–associated synovial inflammation and labral degeneration. The administration of cromolyn can significantly diminish the cascade of inflammatory reactions after labral injury.Clinical Relevance:Our findings support the concept that MC stabilizers may be used as a complementary therapeutic option in the treatment and repair of labral tears.

中文翻译：

---

肥大细胞介导盂唇撕裂后的急性炎症反应，在大鼠模型中可以用色甘酸钠抑制


背景： 关节盂唇损伤被认为是关节疼痛和不适的来源，这可能与导致盂唇组织恶化的炎症反应有关。然而，目前尚不清楚撕裂的盂唇是否会促进肥大细胞 （MC） 活化，从而导致滑膜炎症反应，从而导致盂唇组织变性。目的： 通过监测损伤、缝合修复和 MC 稳定剂给药后滑膜组织和盂唇组织内的 MC 行为和连续炎症反应，确定活化的 MC 对大鼠滑膜炎症反应和随后的盂唇组织变性和肩功能恶化的潜在影响。研究设计： 对照实验室研究。方法： 大鼠右肩 （n = 20） 产生前下关节盂唇撕裂，并使用隧道式缝合技术修复。在一些大鼠腹膜内施用 MC 稳定剂 - 色甘酸钠 （n = 10） 时，滑膜组织炎症反应受到调节。在第 1 周和第 3 周，对 MC 激活、滑膜炎症反应和盂唇变性进行组织学评估。同时，在手术修复前后进行步态分析，以评估损伤和治疗后肩部功能的恶化情况。结果： 盂唇损伤后常驻 MC 脱颗粒 （第 1 周激活 50.48% ± 8.23%） 有助于滑膜组织炎症细胞募集、炎症产物释放、基质金属蛋白酶-13 和随后的盂唇组织细胞外基质变性。 研究发现，使用色甘酸钠（一种 MC 稳定剂）可显着减少损伤介导的炎症反应（炎症细胞浸润和随后的促炎产物分泌）并改善肩部功能恢复。结论： MC 激活是导致盂唇撕裂相关滑膜炎症和盂唇变性的原因。色甘酸钠的给药可以显着减少盂唇损伤后炎症反应的级联反应。临床相关性： 我们的研究结果支持 MC 稳定剂可用作治疗和修复盂唇撕裂的补充治疗选择的概念。