Scanning Electrochemical Microscopy (SECM) has been used as a non-invasive electrochemical technique for studying cellular processes. SECM enables the quantification of cellular metabolites in real-time providing a deeper understanding of cellular responses to external stimuli. SECM imaging of living cells requires maintaining an ideal physiological environment to ensure reliable data collection on cellular reactivity. The cellular response can be directly influenced by physicochemical parameters including cell media composition, temperature and light exposure. This research demonstrates the effect of media composition on the electrochemical current signal of adenocarcinoma cervical cancer (HeLa) cells during SECM measurements using ferrocenemethanol as a redox mediator. Investigated media that are commonly used as electrolyte, are phosphate buffered saline (PBS), and Dulbecco's modified Eagle's medium (DMEM) in the absence and presence of fetal bovine serum (FBS). In addition, this research demonstrates that fluctuating light illumination impacts the stability of the cellular electrochemical current response. Our findings reveal that media composition and illumination are important parameters that must be carefully considered and monitored during SECM live cell imaging.

中文翻译：

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介质成分和光照对扫描电化学显微镜活细胞成像过程中电化学电流响应的影响


扫描电化学显微镜 （SECM） 已被用作研究细胞过程的非侵入性电化学技术。SECM 能够实时定量细胞代谢物，从而更深入地了解细胞对外部刺激的反应。活细胞的 SECM 成像需要保持理想的生理环境，以确保可靠地收集有关细胞反应性的数据。细胞反应可直接受物理化学参数的影响，包括细胞培养基组成、温度和光照。本研究证明了在使用二碳烯醇作为氧化还原介质进行 SECM 测量期间，培养基组成对腺癌宫颈癌 （HeLa） 细胞电化学电流信号的影响。通常用作电解质的研究培养基是磷酸盐缓冲盐水 （PBS） 和在不存在胎牛血清 （FBS） 的情况下的 Dulbecco 改良 Eagle 培养基 （DMEM）。此外，这项研究表明，波动的光照明会影响细胞电化学电流响应的稳定性。我们的研究结果表明，培养基成分和照明是在 SECM 活细胞成像过程中必须仔细考虑和监测的重要参数。