Long-term survival after lung transplantation remains limited by chronic lung allograft dysfunction (CLAD), with 2 main phenotypes: bronchiolitis obliterans syndrome (BOS) and restrictive allograft syndrome (RAS). We aimed to assess CLAD lung allografts using imaging mass cytometry (IMC), a high dimensional tissue imaging system allowing a multiparametric in situ exploration at a single cell level. Four BOS, 4 RAS, and 4 control lung samples were stained with 35 heavy metal-tagged antibodies selected to assess structural and immune proteins of interest. We identified 50 immune and non-immune cell clusters. CLAD lungs had significantly reduced club cells. A Ki67-high basal cell population was mostly present in RAS and in proximity to memory T cells. Memory CD8+ T cells were more frequent in CLAD lungs, regulatory T cells more prominent in RAS. IMC is a powerful technology for detailed cellular analysis within intact organ structures that may shed further light on CLAD mechanisms.

中文翻译：

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使用成像质谱流式细胞术详细描述慢性肺同种异体移植物功能障碍的细胞和空间特征。


肺移植后的长期生存率仍然受到慢性肺同种异体移植物功能障碍 （CLAD） 的限制，有 2 种主要表型：闭塞性细支气管炎综合征 （BOS） 和限制性同种异体移植物综合征 （RAS）。我们旨在使用成像质谱流式细胞术 （IMC） 评估 CLAD 肺同种异体移植物，IMC 是一种高维组织成像系统，允许在单细胞水平进行多参数原位探查。用 35 种重金属标记抗体对 4 个 BOS、4 个 RAS 和 4 个对照肺样本进行染色，以评估感兴趣的结构和免疫蛋白。我们确定了 50 个免疫和非免疫细胞簇。CLAD 肺的棒状细胞显著减少。Ki67 高基础细胞群主要存在于 RAS 中，靠近记忆 T 细胞。记忆 CD8+ T 细胞在 CLAD 肺中更常见，调节性 T 细胞在 RAS 中更突出。IMC 是一种强大的技术，用于对完整器官结构内的详细细胞进行分析，这可能会进一步阐明 CLAD 机制。