Weak protein interactions are associated with a broad array of biological functions and are often implicated in molecular dysfunction accompanying human disease. In addition, these interactions are a critical determinant in the effective manufacturing, stability, and administration of biotherapeutic proteins. Despite their prominence, much remains unknown about how molecular attributes influence the hydrodynamic and thermodynamic contributions to the overall interaction mechanism. To systematically probe these contributions, we have evaluated self-interaction in a diverse set of proteins that demonstrate a broad range of behaviors from attractive to repulsive. Analysis of the composite trending in the data provides a convenient interconversion among interaction parameters measured from the concentration dependence of the molecular weight, diffusion coefficient, and sedimentation coefficient, as well as insight into the relationship between thermodynamic and hydrodynamic interactions. We find relatively good agreement between our data and a model for interacting hard spheres in the range of weak self-association. In addition, we propose an empirically derived, general scaling relationship applicable across a broad range of self-association and repulsive behaviors.

中文翻译：

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蛋白质溶液中热力学和流体动力学相互作用之间的缩放关系


弱蛋白质相互作用与广泛的生物学功能有关，并且通常与人类疾病伴随的分子功能障碍有关。此外，这些相互作用是生物治疗性蛋白质有效生产、稳定性和管理的关键决定因素。尽管它们很突出，但关于分子属性如何影响流体动力学和热力学对整体相互作用机制的贡献，仍有许多未知数。为了系统地探索这些贡献，我们评估了一组不同蛋白质的自我相互作用，这些蛋白质表现出从吸引到排斥的广泛行为。对数据中复合材料趋势的分析提供了从分子量、扩散系数和沉降系数的浓度依赖性测量的相互作用参数之间的便捷相互转换，以及对热力学和流体动力学相互作用之间关系的见解。我们发现我们的数据与在弱自关联范围内相互作用的硬球体的模型之间有相对好的一致性。此外，我们提出了一种经验推导的一般缩放关系，适用于广泛的自关联和排斥行为。