There is increasing interest in exploring how endosymbionts could be useful in pest control, including in aphids, which can carry a diversity of endosymbionts. Endosymbionts often have a large impact on host traits, and their presence can be self‐sustaining. Identifying useful host‐endosymbiont combinations for pest control is facilitated by the transfer of specific endosymbionts into target species, particularly if the species lacks the endosymbiont. Here, we complete a comprehensive literature review, which included 56 relevant papers on endosymbiont transfer experiments in aphids, to uncover factors that might influence transfer success. We then report on our own microinjection attempts of diverse facultative endosymbionts from a range of donor species into three agriculturally important aphid species as recipients: the green peach aphid (Myzus persicae), bird cherry‐oat aphid (Rhopalosiphum padi), and Russian wheat aphid (Diuraphis noxia). Combining this information, we consider reasons that impact the successful establishment of lines carrying transferred endosymbionts. These include a lack of stability in donors, deleterious effects on host fitness, the absence of plant‐based (versus vertical) transmission, high genetic variation in the endosymbiont, and susceptibility of an infection to environmental factors. Taking these factors into account should help in increasing success rates in future introductions.

中文翻译：

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遗传和环境因素对害虫蚜虫内共生体转移成功的影响


人们越来越有兴趣探索内共生体如何用于害虫防治，包括可以携带多种内共生体的蚜虫。内共生体通常对宿主特征有很大影响，并且它们的存在可以自我维持。通过将特定的内共生体转移到目标物种中，特别是在缺乏内共生体的物种中，有助于识别用于害虫防治的有用宿主-内共生体组合。在此，我们完成了全面的文献综述，其中包括 56 篇有关蚜虫内共生体转移实验的相关论文，以揭示可能影响转移成功的因素。然后，我们报告了我们自己将来自一系列供体物种的多种兼性内共生体显微注射到作为受体的三种农业上重要的蚜虫物种中的尝试：绿桃蚜虫（Myzus persicae）、鸟樱桃燕麦蚜虫（Rhopalosiphum padi）和俄罗斯小麦蚜虫（Diuraphis noxia）。结合这些信息，我们考虑了影响成功建立携带转移内共生体的细胞系的原因。这些包括供体缺乏稳定性、对宿主适应性的有害影响、缺乏基于植物的（相对于垂直）传播、内共生体的高遗传变异以及对环境因素感染的易感性。考虑到这些因素应该有助于提高未来介绍的成功率。