Nature Structural & Molecular Biology ( IF 12.5 ) Pub Date : 2024-10-01 , DOI: 10.1038/s41594-024-01394-4 Richard D. Schargel, M. Zuhaib Qayyum, Ajay Singh Tanwar, Ravi C. Kalathur, Elizabeth H. Kellogg
RNA-guided endonucleases, once thought to be exclusive to prokaryotes, have been recently identified in eukaryotes and are called Fanzors. They are classified into two clades, Fanzor1 and Fanzor2. Here we present the cryo-electron microscopy structure of Acanthamoeba polyphaga mimivirus Fanzor2, revealing its ωRNA architecture, active site and features involved in transposon-adjacent motif recognition. A comparison to Fanzor1 and TnpB structures highlights divergent evolutionary paths, advancing our understanding of RNA-guided endonucleases.
中文翻译:
Fanzor2 的结构揭示了对 TnpB 超家族进化的见解
RNA 引导的核酸内切酶曾经被认为是原核生物独有的,最近在真核生物中被发现,被称为 Fanzors。它们分为两个分支,Fanzor1 和 Fanzor2。在这里,我们展示了棘阿米巴多噬菌模拟病毒 Fanzor2 的冷冻电子显微镜结构,揭示了其 ωRNA 结构、活性位点和参与转座子相邻基序识别的特征。与 Fanzor1 和 TnpB 结构的比较突出了不同的进化路径,促进了我们对 RNA 引导的核酸内切酶的理解。