Iron deficiency is a common nutritional issue that seriously affects male reproductive health. Lotus root polysaccharide iron (LRPF), a novel nutritional supplement, may ameliorate the damage caused by iron deficiency, however, the mechanism is unclear. In this study, we comprehensively determined the benefits of LRPF on reproduction in iron-deficient mice by integrating transcriptomics, microbiomics and serum metabolomics. Microbiomics showed that LRPF could restore changes to the intestinal microbiota caused by iron deficiency. Metabolomics results showed that LRPF stabilised steroid hormone and fatty acid metabolism in iron-deficient mice, reduced the content of ethyl chrysanthemumate (EC) and ameliorated the reproductive impairment. The transcriptomic analysis showed that LRPF regulated steroid hormone synthesis and the peroxisome proliferator-activated receptor (PPAR) signalling pathway in iron-deficient mice. In vitro experiments showed that LRPF could promote steroid hormone synthesis in Leydig cells by activating PPARγ. In conclusion, this study highlights the advantage of LRPF to improve testicular development.

中文翻译：

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多组学方法表明，莲藕多糖铁通过激活 PPARγ 促进类固醇激素合成，改善缺铁引起的睾丸损伤。


缺铁是一种常见的营养问题，严重影响男性生殖健康。莲藕多糖铁（LRPF）是一种新型营养补充剂，可能改善缺铁造成的损害，但其机制尚不清楚。在本研究中，我们通过整合转录组学、微生物组学和血清代谢组学，全面确定了 LRPF 对缺铁小鼠生殖的益处。微生物组学表明 LRPF 可以恢复因缺铁引起的肠道微生物群的变化。代谢组学结果表明，LRPF 可稳定缺铁小鼠的类固醇激素和脂肪酸代谢，降低菊酸乙酯 (EC) 含量，改善生殖障碍。转录组分析表明，LRPF 调节缺铁小鼠的类固醇激素合成和过氧化物酶体增殖物激活受体 (PPAR) 信号通路。体外实验表明，LRPF可以通过激活PPARγ促进Leydig细胞中类固醇激素的合成。总之，本研究强调了 LRPF 在改善睾丸发育方面的优势。