Oils extracted from natural plants are valuable resources because of their diverse biocidal activity against microorganisms. This paper reports, for the first time, supercritical CO2 extraction of oil from Korarima (Aframomum corrorima) seeds and its antibacterial activity evaluation. The influences of pressure, temperature, and extraction time on the yield of supercritical CO2-extracted oil (scCO2-extracted oil) were optimized using the Response Surface Methodology. Under the optimal extraction conditions of 46 °C, 15.5 MPa, and 147 min, the extraction yield reached 1.60±0.04 %, consistent with the theoretical value of 1.58 %. The compositions of the scCO2-extracted oil were identified by GC-MS, primarily consisting of nerolidol (33.97–41.20 %), geraniol (23.08–24.50 %), and α-terpinene (8.90–9.06 %). The oil exhibited significant antibacterial activity against Staphylococcus. aureus with a minimum inhibitory concentration of 1 mg·mL−1. This study highlights the potential applications of supercritical CO2 extraction technology for extracting Korarima seed oil and its use as an antibacterial ingredient in cosmetics and pharmaceuticals.

中文翻译：

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科拉里马 （Aframomum corrorima） 种子油超临界二氧化碳提取的响应面优化及其抗菌活性评价


从天然植物中提取的油是宝贵的资源，因为它们对微生物具有多种杀菌活性。本文首次报道了 Korarima （Aframomum corrorima） 种子中油的超临界 CO2 萃取及其抗菌活性评价。使用响应面法优化了压力、温度和提取时间对超临界 CO2 萃取油（scCO2 萃取油）产量的影响。在46 °C、15.5 MPa、147 min的最佳提取条件下，提取率达到1.60±0.04 %，与理论值1.58 %一致。通过 GC-MS 鉴定 scCO2 提取的油的成分，主要由橙花醇 （33.97-41.20 %）、香叶醇 （23.08-24.50 %） 和 α-萜品烯 （8.90-9.06 %） 组成。该油对葡萄球菌表现出显着的抗菌活性。金黄色葡萄球菌的最小抑菌浓度为 1 mg·mL-1。本研究重点介绍了超临界 CO2 萃取技术在提取 Korarima 籽油方面的潜在应用，以及其在化妆品和药品中用作抗菌成分。