Pathogenic Escherichia coli, which will easily cause environmental pollution, is a potentially hazardous microorganism that enters the environment through various routes such as biological feces. The presence of this bacterium poses a serious health risk, especially in water sources. When consuming water containing excessive amounts of pathogenic Escherichia coli, both humans and animals may experience severe health issues, such as diarrhea and gastrointestinal infections. However, current treatment methods for this bacterium are not ideal, and traditional means sometimes struggle to completely eliminate these stubborn microorganisms. In recent years, the scientific community has been exploring new sterilization techniques to address this challenge without causing secondary environmental pollution. Among them, pulsed electric field (PEF) technology has garnered significant attention. By applying high-intensity electric field pulses, PEF technology can have a fatal impact on microbial cells in a very short period of time. Studies have shown that this technology can cause cell membrane perforation, destroying the integrity of the cell and leading to cell death. This method is not only capable of decomposing organic pollutants, but also has a significant bactericidal effect. Nevertheless, despite the theoretical potential of PEF technology, there is still relatively little research on its application, especially in terms of treating pathogenic Escherichia coli. Further studies and experimental verification are needed. This article investigates the bioelectromagnetic effects of electric pulses on pathogenic Escherichia coli in sewage through a self-developed pulse source device (capable of providing a voltage range from 1.7 kV to 2.2 kV, with a rise time of 190 ps and a maximum repetition rate of 20 kHz). The feasibility of using a pulsed electric field to purify water bodies is verified in this report.

中文翻译：

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一种基于雪崩晶体管的 Marx 电路脉冲发生器，具有亚纳秒、高频和高压功能，用于致病性大肠杆菌消融术


致病性大肠埃希菌，容易造成环境污染，是一种具有潜在危害的微生物，通过生物粪便等多种途径进入环境。这种细菌的存在会带来严重的健康风险，尤其是在水源中。当饮用含有过量致病性大肠杆菌的水时，人类和动物都可能会出现严重的健康问题，例如腹泻和胃肠道感染。然而，目前针对这种细菌的治疗方法并不理想，传统方法有时难以完全消除这些顽固的微生物。近年来，科学界一直在探索新的灭菌技术，以应对这一挑战，而不会造成二次环境污染。其中，脉冲电场 （PEF） 技术引起了广泛关注。通过施加高强度电场脉冲，PEF 技术可以在很短的时间内对微生物细胞产生致命影响。研究表明，这项技术会导致细胞膜穿孔，破坏细胞的完整性并导致细胞死亡。该方法不仅能分解有机污染物，而且杀菌效果显著。然而，尽管 PEF 技术具有理论潜力，但对其应用的研究仍然相对较少，尤其是在治疗致病性大肠杆菌方面。需要进一步的研究和实验验证。本文通过自主研发的脉冲源装置（能够提供 1.7 kV 至 2 的电压范围）研究了电脉冲对污水中致病性大肠杆菌的生物电磁效应。2 kV，上升时间为 190 ps，最大重复频率为 20 kHz）。本报告验证了使用脉冲电场净化水体的可行性。