AimPeri‐implant mucositis, a dysbiosis‐driven inflammatory disease, is a precursor to peri‐implantitis, underscoring the need for early disease management. Therefore, we investigated the efficacy of glycine powder in resolving clinical inflammation and restoring host‐microbial homeostasis.MethodsThirty subjects were randomized to receive either glycine powder air‐abrasive debridement or ultrasonic instrumentation. Clinical parameters (probe depth [PD], modified Sulcular Bleeding Index [mSBI], modified Plaque Index [mPlI]), biofilm and peri‐implant crevicular fluid were collected at baseline and at 1‐day, 1‐, 3‐, 6‐weeks and 3‐ and 6‐months post‐therapy. Microbial recolonization was examined using 16S rDNA sequencing and immune response was semi‐quantified using a bead‐based 17‐plex microarray.ResultsAt 6‐months, both groups demonstrated non‐significant reductions in mSBI when compared to baseline (p > 0.05, Wald test, mixed model for repeated measures). However, mSBI and PD decreased in the test group from week‐1 to 3‐months, while control group decreased at 1‐ and 3‐weeks only. mSBI was lower in the test group when compared to controls from Week‐1 to 3‐months, while PD differed between groups at 6 weeks and 3‐months. Glycine group demonstrated significant microbial shifts after 24‐h, increases in species richness and health‐compatible species, and loss of pathobionts (p < 0.001, Dunn test). Pro‐inflammatory cytokines decreased from 1‐ to 6‐weeks or 3‐months (p < 0.05, Wald test). Comparable results were obtained in the ultrasonic group at 3‐weeks and sustained over 6‐weeks post‐therapy.ConclusionsGlycine therapy leads to early and sustained change in host‐microbial interactions when compared to ultrasonics, however, the changes wrought by both therapies were sustained for a maximum of 3 months.Trial RegistrationClinicalTrials.gov identifier: NCT05810558

中文翻译：

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甘氨酸粉末空气磨料清创术对种植体周围粘膜炎的影响：临床、微生物和免疫学变化的随机对照试验


Aim种植体周围粘膜炎是一种生态失调驱动的炎症性疾病，是种植体周围炎的前兆，强调了早期疾病管理的必要性。因此，我们研究了甘氨酸粉在解决临床炎症和恢复宿主微生物稳态方面的疗效。方法将 30 名受试者随机分配接受甘氨酸粉末空气磨料清创术或超声器械治疗。在基线和治疗后 1 天、 1 天、 3 周、 6 周以及 3 个月和 6 个月收集临床参数 （探针深度 [PD] 、改良沟出血指数 [mSBI]、改良斑块指数 [mPlI]）、生物膜和种植体周围牙槽液。使用 16S rDNA 测序检查微生物再定植，并使用基于磁珠的 17 重微阵列半定量免疫反应。结果在 6 个月时，与基线相比，两组的 mSBI 均未显著降低 （p > 0.05，Wald 检验，重复测量的混合模型）。然而，测试组的 mSBI 和 PD 在第 1 周到 3 个月有所下降，而对照组仅在第 1 周和 3 周下降。与对照组相比，第 1 周至 3 个月的 mSBI 较低，而 PD 在 6 周和 3 个月时各组不同。甘氨酸组在 24 小时后表现出显着的微生物变化，物种丰富度和健康相容物种的增加，以及病原体的损失 （p < 0.001，Dunn 检验）。促炎细胞因子从 1 周到 6 周或 3 个月下降 （p < 0.05，Wald 检验）。超声组在治疗后 3 周获得相似的结果，并持续治疗后 6 周以上。结论与超声相比，甘氨酸疗法导致宿主与微生物相互作用的早期和持续变化，然而，两种疗法造成的变化最多持续 3 个月。试验注册临床试验。gov 标识符：NCT05810558