AimPeri‐implant mucositis, a dysbiosis‐driven inflammatory disease, is a precursor to peri‐implantitis, underscoring the need for early disease management. Therefore, we investigated the efficacy of glycine powder in resolving clinical inflammation and restoring host‐microbial homeostasis.MethodsThirty subjects were randomized to receive either glycine powder air‐abrasive debridement or ultrasonic instrumentation. Clinical parameters (probe depth [PD], modified Sulcular Bleeding Index [mSBI], modified Plaque Index [mPlI]), biofilm and peri‐implant crevicular fluid were collected at baseline and at 1‐day, 1‐, 3‐, 6‐weeks and 3‐ and 6‐months post‐therapy. Microbial recolonization was examined using 16S rDNA sequencing and immune response was semi‐quantified using a bead‐based 17‐plex microarray.ResultsAt 6‐months, both groups demonstrated non‐significant reductions in mSBI when compared to baseline (p > 0.05, Wald test, mixed model for repeated measures). However, mSBI and PD decreased in the test group from week‐1 to 3‐months, while control group decreased at 1‐ and 3‐weeks only. mSBI was lower in the test group when compared to controls from Week‐1 to 3‐months, while PD differed between groups at 6 weeks and 3‐months. Glycine group demonstrated significant microbial shifts after 24‐h, increases in species richness and health‐compatible species, and loss of pathobionts (p < 0.001, Dunn test). Pro‐inflammatory cytokines decreased from 1‐ to 6‐weeks or 3‐months (p < 0.05, Wald test). Comparable results were obtained in the ultrasonic group at 3‐weeks and sustained over 6‐weeks post‐therapy.ConclusionsGlycine therapy leads to early and sustained change in host‐microbial interactions when compared to ultrasonics, however, the changes wrought by both therapies were sustained for a maximum of 3 months.Trial RegistrationClinicalTrials.gov identifier: NCT05810558

中文翻译：

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甘氨酸粉末空气磨料清创术对种植体周围粘膜炎的影响：临床、微生物和免疫学变化的随机对照试验


目的种植体周围粘膜炎是一种生态失调驱动的炎症性疾病，是种植体周围炎的先兆，强调了早期疾病管理的必要性。因此，我们研究了甘氨酸粉末在解决临床炎症和恢复宿主微生物稳态方面的功效。方法30 名受试者随机接受甘氨酸粉末空气研磨清创术或超声仪器治疗。在基线和第 1 天、第 1 天、第 3 天、第 6 天收集临床参数（探头深度 [PD]、改良的龈沟出血指数 [mSBI]、改良的斑块指数 [mPlI]）、生物膜和种植体周围的沟液。治疗后几周、3 个月和 6 个月。使用 16S rDNA 测序检查微生物重新定殖，并使用基于珠子的 17 重微阵列对免疫反应进行半定量。结果在 6 个月时，与基线相比，两组的 mSBI 均没有显着降低（p > 0.05，Wald）测试，重复测量的混合模型）。然而，测试组的 mSBI 和 PD 从第 1 周到第 3 个月有所下降，而对照组仅在第 1 周和第 3 周有所下降。从第 1 周到第 3 个月，测试组的 mSBI 低于对照组，而组间的 PD 在第 6 周和第 3 个月时有所不同。甘氨酸组在 24 小时后表现出显着的微生物变化、物种丰富度和健康相容性物种的增加以及病原体的损失（p < 0.001，Dunn 检验）。促炎细胞因子从 1 周减少到 6 周或 3 个月（p < 0.05，Wald 检验）。超声组在治疗 3 周时获得了可比较的结果，并且在治疗后持续了 6 周以上。结论与超声波相比，甘氨酸疗法会导致宿主-微生物相互作用的早期且持续的变化，然而，两种疗法造成的变化最多持续 3 个月。试验注册临床试验。政府标识符：NCT05810558