Acute leukemia is characterized by abnormal white blood cell proliferation with rapid onset and severe complications. Natural compounds, which are alternative treatments, are widely used in cancer treatment. Piperine, an alkaloid compound from black pepper, exerts anticancer effects through the cell death signaling pathway. Autophagy and senescence signaling pathways are considered target signaling pathways for cancer treatment. In this study, we investigated the effects of piperine via autophagy and senescence signaling pathways in NB4 and MOLT-4 cells. The MTT assay results demonstrated that piperine significantly decreased the viability of NB4 and MOLT-4 cells. Piperine induced autophagy by increasing LC3, Beclin-1 and ULK1 and decreasing mTOR and NF-κB1 expression in NB4 and MOLT-4 cells. In addition, piperine increased senescence-associated beta-galactosidase fluorescence intensity by increasing p21 and IL-6 expression while decreasing CDK2 expression in NB4 and MOLT-4 cells. In conclusion, our study provides additional information about the induction of autophagy and senescence by piperine in acute leukemia.

急性白血病的特点是白细胞异常增殖，发病迅速，并发症严重。天然化合物作为替代疗法，广泛用于癌症治疗。胡椒碱是一种来自黑胡椒的生物碱化合物，通过细胞死亡信号通路发挥抗癌作用。自噬和衰老信号通路被认为是癌症治疗的靶信号通路。在这项研究中，我们研究了胡椒碱通过自噬和衰老信号通路对 NB4 和 MOLT-4 细胞的影响。 MTT测定结果表明胡椒碱显着降低NB4和MOLT-4细胞的活力。胡椒碱通过增加 NB4 和 MOLT-4 细胞中 LC3、Beclin-1 和 ULK1 的表达并降低 mTOR 和 NF-κB1 的表达来诱导自噬。此外，胡椒碱通过增加 NB4 和 MOLT-4 细胞中 p21 和 IL-6 的表达同时减少 CDK2 的表达来增加衰老相关的 β-半乳糖苷酶荧光强度。总之，我们的研究提供了有关胡椒碱在急性白血病中诱导自噬和衰老的更多信息。