Invasiveness and epithelial–mesenchymal transition (EMT) are main patterns of metastatic disease, which is the major cause cancer‐related mortality in human malignant melanoma cells. Tea and its consumption extract are associated with a lower risk of several types of cancer and have anti‐inflammatory and antioxidative biological effects. However, the anti‐EMT and anti‐cancer stemness effect of black tea ethanol extracts (BTEE) in human melanoma remain poorly understood. In this study, the effects of BTEE‐reduced invasiveness, EMT, and cancer stemness were evaluated in human A 375 and A2058 melanoma cells. BTEE inhibited the activity of u‐PA, migration, and invasiveness by repressing p‐FAK signaling pathway. BTEE affected the EMT by downregulating the expression of β‐catenin, N‐cadherin, fibronectin, vimentin, and Twist‐1. BTEE also reduced tumor necrosis factor‐alpha (TNF‐α)‐induced invasiveness and cancer stemness characteristics in vitro. The growth of melanoma in nude mice xenograft model showed that BTEE suppressed A 375 tumor growth in vivo.

中文翻译：

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红茶抑制人恶性黑色素瘤细胞的侵袭性并逆转 TNF-α 诱导的侵袭性和细胞干性


侵袭性和上皮间质转化（EMT）是转移性疾病的主要模式，是人类恶性黑色素瘤细胞癌症相关死亡的主要原因。茶及其提取物可降低多种癌症的风险，并具有抗炎和抗氧化的生物作用。然而，红茶乙醇提取物 (BTEE) 对人类黑色素瘤的抗 EMT 和抗癌干细胞作用仍知之甚少。在这项研究中，在人类 A 375 和 A2058 黑色素瘤细胞中评估了 BTEE 降低侵袭性、EMT 和癌症干性的影响。 BTEE 通过抑制 p-FAK 信号通路来抑制 u-PA 的活性、迁移和侵袭。 BTEE 通过下调 β-连环蛋白、N-钙粘蛋白、纤连蛋白、波形蛋白和 Twist-1 的表达来影响 EMT。 BTEE 还在体外降低了肿瘤坏死因子-α (TNF-α) 诱导的侵袭性和癌症干性特征。裸鼠异种移植模型中黑色素瘤的生长表明BTEE抑制体内A 375肿瘤的生长。